{"title":"治疗性纳米制剂在基于胶原蛋白的三维结直肠癌体外模型中的疗效","authors":"Victor Lopez Davila","doi":"10.4172/2157-7552.S1.022","DOIUrl":null,"url":null,"abstract":"Methods: AZD6244-containing GCPQ micelles and DOPE/DC-cholesterol liposomes were fabricated using sonication and extrusion methods respectively. These formulations were tested together with the free drug in HCT116 cell monolayers and a collagen-based 3D cancer model to determine differences in efficacy between both models. Fluorescent nanoparticles were used to determine their ability to penetrate the scaffold and reach its core.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"73 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2015-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":"{\"title\":\"Efficacy of therapeutic nanoformulations in a collagen-based three-dimensional colorectal cancer in vitro model\",\"authors\":\"Victor Lopez Davila\",\"doi\":\"10.4172/2157-7552.S1.022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Methods: AZD6244-containing GCPQ micelles and DOPE/DC-cholesterol liposomes were fabricated using sonication and extrusion methods respectively. These formulations were tested together with the free drug in HCT116 cell monolayers and a collagen-based 3D cancer model to determine differences in efficacy between both models. Fluorescent nanoparticles were used to determine their ability to penetrate the scaffold and reach its core.\",\"PeriodicalId\":17539,\"journal\":{\"name\":\"Journal of Tissue Science and Engineering\",\"volume\":\"73 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Tissue Science and Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/2157-7552.S1.022\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Tissue Science and Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2157-7552.S1.022","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
摘要
方法:采用超声法制备含GCPQ胶束的azd6244，采用挤压法制备含DOPE/ dc -胆固醇脂质体。这些配方与游离药物一起在HCT116细胞单层和基于胶原蛋白的3D癌症模型中进行测试，以确定两种模型之间的疗效差异。荧光纳米颗粒被用来测定它们穿透支架并到达其核心的能力。
Efficacy of therapeutic nanoformulations in a collagen-based three-dimensional colorectal cancer in vitro model
Methods: AZD6244-containing GCPQ micelles and DOPE/DC-cholesterol liposomes were fabricated using sonication and extrusion methods respectively. These formulations were tested together with the free drug in HCT116 cell monolayers and a collagen-based 3D cancer model to determine differences in efficacy between both models. Fluorescent nanoparticles were used to determine their ability to penetrate the scaffold and reach its core.
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