牛真核翻译起始因子4E (eIF-4E)的克隆及其在不同生理阶段牛乳腺中的表达

Ezhou L. Long, A. Capuco, Xin Zhao
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引用次数: 8

摘要

克隆了牛eIF-4E cDNA,并对其在不同生理阶段牛乳腺中的表达进行了研究。牛eIF-4E cDNA和蛋白序列与其他哺乳动物高度同源。通过Northern blot分析,我们没有检测到eIF-4E在青春期前的牛乳腺中表达,而在第三发情周期的母牛乳腺组织中观察到低水平的eIF-4E mRNA。泌乳乳腺中eIF-4E mRNA水平显著高于第三发情周期母牛乳腺组织。不同生理阶段牛乳腺组织中eIF-4E表达的变化表明eIF-4E参与乳腺发育。哺乳期eIF-4E表达升高可能与某些mRNA翻译增加或整体蛋白质合成加速有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cloning of Bovine Eukaryotic Translation Initiation Factor 4E (eIF-4E) and Its Expression in the Bovine Mammary Gland at Different Physiological Stages
Bovine eIF-4E cDNA was cloned and its expression in the bovine mammary gland at distinct physiological stages was investigated. Bovine eIF-4E cDNA and protein sequences are highly homologous to those from other mammals. Using Northern blot analysis, we did not detect eIF-4E expression in the prepubertal bovine mammary gland, whereas a low level of eIF-4E mRNA was observed in the mammary tissues of heifers during the third estrous cycle. The eIF-4E mRNA level was significantly higher in the lactating mammary gland compared to the mammary tissues obtained from heifers during the third estrous cycle. Alteration of eIF-4E expression in bovine mammary tissues during different physiological stages indicates the involvement of eIF-4E in mammary development. Elevated eIF-4E expression during lactation may be related to increased translation of certain mRNA or the acceleration of overall protein synthesis.
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