P. Dimayuga, B. Cercek, S. Oguchi, G. N. Fredrikson, J. Yano, P. Shah, S. Jovinge, J. Nilsson
{"title":"过继性b细胞转移对Rag-1敲除小鼠动脉损伤诱导的新内膜形成的抑制作用","authors":"P. Dimayuga, B. Cercek, S. Oguchi, G. N. Fredrikson, J. Yano, P. Shah, S. Jovinge, J. Nilsson","doi":"10.1161/01.ATV.0000012455.62765.BF","DOIUrl":null,"url":null,"abstract":"We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020±0.0160 [n=8] versus 0.0049±0.0022 [n=8] mm2, respectively;P <0.05) and in western-type diet–fed Rag-1 KO mice compared with WT mice (0.0312±0.0174 [n=7] versus 0.0050±0.0028 [n=6] mm2, respectively;P <0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed normal chow) reduced neointimal formation compared with the effect in unreconstituted Rag-1 KO mice (0.0076±0.0039 [n=9] versus 0.020±0.0160 [n=8] mm2, respectively;P <0.05). Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed a western diet) reduced neointimal formation compared the effect in Rag-1 KO mice (0.0087±0.0037 [n=8] versus 0.0312±0.0174 [n=7] mm2, respectively;P <0.05). Injured carotid arteries from reconstituted Rag-1 KO mice had detectable IgM and IgG, indicating viable transfer of B cells. The results suggest that B cells modulate the response to arterial injury.","PeriodicalId":8418,"journal":{"name":"Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2002-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"53","resultStr":"{\"title\":\"Inhibitory Effect on Arterial Injury-Induced Neointimal Formation by Adoptive B-Cell Transfer in Rag-1 Knockout Mice\",\"authors\":\"P. Dimayuga, B. Cercek, S. Oguchi, G. N. Fredrikson, J. Yano, P. Shah, S. Jovinge, J. Nilsson\",\"doi\":\"10.1161/01.ATV.0000012455.62765.BF\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020±0.0160 [n=8] versus 0.0049±0.0022 [n=8] mm2, respectively;P <0.05) and in western-type diet–fed Rag-1 KO mice compared with WT mice (0.0312±0.0174 [n=7] versus 0.0050±0.0028 [n=6] mm2, respectively;P <0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed normal chow) reduced neointimal formation compared with the effect in unreconstituted Rag-1 KO mice (0.0076±0.0039 [n=9] versus 0.020±0.0160 [n=8] mm2, respectively;P <0.05). Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed a western diet) reduced neointimal formation compared the effect in Rag-1 KO mice (0.0087±0.0037 [n=8] versus 0.0312±0.0174 [n=7] mm2, respectively;P <0.05). Injured carotid arteries from reconstituted Rag-1 KO mice had detectable IgM and IgG, indicating viable transfer of B cells. The results suggest that B cells modulate the response to arterial injury.\",\"PeriodicalId\":8418,\"journal\":{\"name\":\"Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"53\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1161/01.ATV.0000012455.62765.BF\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Arteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1161/01.ATV.0000012455.62765.BF","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 53
摘要
我们研究了免疫缺陷rag1敲除(KO)小鼠动脉损伤后b细胞重构的影响。21 d后,正常饲料喂养的Rag-1 KO小鼠的新生内膜形成比野生型(WT)小鼠增加4 ~ 5倍(分别为0.020±0.0160 [n=8]和0.0049±0.0022 [n=8] mm2, P <0.05),西式饲料喂养的Rag-1 KO小鼠的新生内膜形成比野生型(WT)小鼠增加4 ~ 5倍(分别为0.0312±0.0174 [n=7]和0.0050±0.0028 [n=6] mm2, P <0.05)。为了研究B细胞在损伤反应中的作用,我们用来自WT小鼠脾脏的B细胞重建了rag1 KO小鼠,供体和受体的饮食相同。与未重组的rag1 - KO小鼠相比,用WT小鼠的B细胞重组rag1 - KO小鼠(均饲喂正常食物)减少了新内膜的形成(分别为0.0076±0.0039 [n=9]和0.020±0.0160 [n=8] mm2, P <0.05)。用WT小鼠的B细胞重建Rag-1 KO小鼠(均饲喂西方饮食),与Rag-1 KO小鼠相比,新内膜形成减少(分别为0.0087±0.0037 [n=8]和0.0312±0.0174 [n=7] mm2, P <0.05)。重组Rag-1 KO小鼠损伤颈动脉中检测到IgM和IgG,表明B细胞有活力转移。结果提示B细胞可调节动脉损伤反应。
Inhibitory Effect on Arterial Injury-Induced Neointimal Formation by Adoptive B-Cell Transfer in Rag-1 Knockout Mice
We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020±0.0160 [n=8] versus 0.0049±0.0022 [n=8] mm2, respectively;P <0.05) and in western-type diet–fed Rag-1 KO mice compared with WT mice (0.0312±0.0174 [n=7] versus 0.0050±0.0028 [n=6] mm2, respectively;P <0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed normal chow) reduced neointimal formation compared with the effect in unreconstituted Rag-1 KO mice (0.0076±0.0039 [n=9] versus 0.020±0.0160 [n=8] mm2, respectively;P <0.05). Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed a western diet) reduced neointimal formation compared the effect in Rag-1 KO mice (0.0087±0.0037 [n=8] versus 0.0312±0.0174 [n=7] mm2, respectively;P <0.05). Injured carotid arteries from reconstituted Rag-1 KO mice had detectable IgM and IgG, indicating viable transfer of B cells. The results suggest that B cells modulate the response to arterial injury.