杜鹃杂种多倍体的离体诱导。

Mededelingen Pub Date : 2002-02-01 DOI:10.17660/ActaHortic.2002.572.4

T. Eeckhout, G. Samyn, E. van Bockstaele

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引用次数: 28

摘要

对三种四倍体杜鹃花杂交的体外诱导方案进行了评价。最成功的方法是每天在幼苗的顶端分生组织上施用有丝分裂毒。然而，这种技术是相当劳动密集型的。在富含这些化合物的培养基上直接播种需要较少的工作，但效率较低。有丝分裂抑制剂，当添加到增殖培养基时，诱导现有基因型的倍体嵌合体。

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In vitro polyploidy induction in Rhododendron simsii-hybrids.

Three in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was rather labour intensive. Direct sowing on media enriched with these compounds required less work but was less efficient. Mitotic inhibitors, when added to multiplication media, induced ploidy chimeras of existing genotypes.

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Mededelingen

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