{"title":"弓形虫抗原免疫小鼠的延迟二次免疫反应及其对弓形虫的保护作用","authors":"Kenyichi Yano , Toshio Nakabayashi","doi":"10.1016/S0174-3031(84)80016-5","DOIUrl":null,"url":null,"abstract":"<div><p>Primary immune response by mice immunized with <em>Toxoplasma</em> peritoneal exudate (PE) was produced slowly at a low level.</p><p>To induce high <em>Toxoplasma</em> antibodies, mice should be boostered after the primary immune response reached the peak in 4 weeks or later.</p><p>Immunization of mice with PE alone in 4 weeks could suppress the growth of <em>Toxoplasma</em> but could not accomplish protection. Two of 9 mice immunized with PE in complete Freund’s adjuvant (CFA), and 16 of 27 mice immunized with PE containing <em>Toxoplasma</em> (ToxoPE) in CFA were protected against a challenge of 1 × 10<sup>3</sup><em>Toxoplasma</em>, RH strain. The mice surviving to the challenge showed complete protection against a rechallenge of higher dose, 1 × 10<sup>6</sup> RH strain.</p><p>Their ascites were very high in LA titer and would be a great source for obtaining high titer antibodies to <em>Toxoplasma</em> without contamination of host serum components. Biological characters of <em>Toxoplasma</em>, RH strain, in chronic infection of mice are discussed.</p></div>","PeriodicalId":79282,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...","volume":"256 3","pages":"Pages 381-389"},"PeriodicalIF":0.0000,"publicationDate":"1984-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-3031(84)80016-5","citationCount":"1","resultStr":"{\"title\":\"Delayed secondary immune response in mice immunized with toxoplasma antigens and its effect for protection against toxoplasma gondii\",\"authors\":\"Kenyichi Yano , Toshio Nakabayashi\",\"doi\":\"10.1016/S0174-3031(84)80016-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Primary immune response by mice immunized with <em>Toxoplasma</em> peritoneal exudate (PE) was produced slowly at a low level.</p><p>To induce high <em>Toxoplasma</em> antibodies, mice should be boostered after the primary immune response reached the peak in 4 weeks or later.</p><p>Immunization of mice with PE alone in 4 weeks could suppress the growth of <em>Toxoplasma</em> but could not accomplish protection. Two of 9 mice immunized with PE in complete Freund’s adjuvant (CFA), and 16 of 27 mice immunized with PE containing <em>Toxoplasma</em> (ToxoPE) in CFA were protected against a challenge of 1 × 10<sup>3</sup><em>Toxoplasma</em>, RH strain. The mice surviving to the challenge showed complete protection against a rechallenge of higher dose, 1 × 10<sup>6</sup> RH strain.</p><p>Their ascites were very high in LA titer and would be a great source for obtaining high titer antibodies to <em>Toxoplasma</em> without contamination of host serum components. Biological characters of <em>Toxoplasma</em>, RH strain, in chronic infection of mice are discussed.</p></div>\",\"PeriodicalId\":79282,\"journal\":{\"name\":\"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...\",\"volume\":\"256 3\",\"pages\":\"Pages 381-389\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0174-3031(84)80016-5\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0174303184800165\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0174303184800165","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Delayed secondary immune response in mice immunized with toxoplasma antigens and its effect for protection against toxoplasma gondii
Primary immune response by mice immunized with Toxoplasma peritoneal exudate (PE) was produced slowly at a low level.
To induce high Toxoplasma antibodies, mice should be boostered after the primary immune response reached the peak in 4 weeks or later.
Immunization of mice with PE alone in 4 weeks could suppress the growth of Toxoplasma but could not accomplish protection. Two of 9 mice immunized with PE in complete Freund’s adjuvant (CFA), and 16 of 27 mice immunized with PE containing Toxoplasma (ToxoPE) in CFA were protected against a challenge of 1 × 103Toxoplasma, RH strain. The mice surviving to the challenge showed complete protection against a rechallenge of higher dose, 1 × 106 RH strain.
Their ascites were very high in LA titer and would be a great source for obtaining high titer antibodies to Toxoplasma without contamination of host serum components. Biological characters of Toxoplasma, RH strain, in chronic infection of mice are discussed.
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