肺炎衣原体和噬菌体衣原体之间水平基因转移的证据

A. Rosenwald, B. Murray, Theodore Toth, R. Madupu, A. Kyrillos, Gaurav S. Arora
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引用次数: 14

摘要

感染衣原体的噬菌体是微病毒科的成员,特别是Gokushovirinae亚科,是小的(4.5-5 kb)单链圆环,具有8-10个开放阅读框,类似于大肠杆菌噬菌体φX174。利用GenBank中发现的序列信息,我们检测了肺炎衣原体和感染衣原体的噬菌体的相关基因。这5个完全测序的肺炎球菌菌株含有一个与噬菌体基因同源的基因,该基因被注释为假定的复制起始蛋白(PRIP,也称为VP4),这是迄今为止在衣原体科的任何其他测序成员中未发现的。感染考拉的肺炎球菌菌株LPCoLN还含有另一个与次要衣壳蛋白基因VP3衍生的噬菌体序列同源的区域。在系统发育上,噬菌体的PRIP序列比细菌的更多样化;然而,细菌序列和噬菌体序列在各自的分支中都聚集在一起。最后,我们发现了另一个微病毒科噬菌体相关基因,主要的衣壳蛋白基因VP1在许多其他细菌物种和2种真核生物,林地草莓和线虫中存在的证据。因此,我们不仅在各种原核生物中而且在真核生物物种中发现了大量与微病毒科噬菌体中发现的基因相关的DNA序列的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evidence for horizontal gene transfer between Chlamydophila pneumoniae and Chlamydia phage
Chlamydia-infecting bacteriophages, members of the Microviridae family, specifically the Gokushovirinae subfamily, are small (4.5–5 kb) single-stranded circles with 8–10 open-reading frames similar to E. coli phage φX174. Using sequence information found in GenBank, we examined related genes in Chlamydophila pneumoniae and Chlamydia-infecting bacteriophages. The 5 completely sequenced C. pneumoniae strains contain a gene orthologous to a phage gene annotated as the putative replication initiation protein (PRIP, also called VP4), which is not found in any other members of the Chlamydiaceae family sequenced to date. The C. pneumoniae strain infecting koalas, LPCoLN, in addition contains another region orthologous to phage sequences derived from the minor capsid protein gene, VP3. Phylogenetically, the phage PRIP sequences are more diverse than the bacterial PRIP sequences; nevertheless, the bacterial sequences and the phage sequences each cluster together in their own clade. Finally, we found evidence for another Microviridae phage-related gene, the major capsid protein gene, VP1 in a number of other bacterial species and 2 eukaryotes, the woodland strawberry and a nematode. Thus, we find considerable evidence for DNA sequences related to genes found in bacteriophages of the Microviridae family not only in a variety of prokaryotic but also eukaryotic species.
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