三叶草(葫芦科)消灭人乳腺癌、结肠癌和肺癌细胞:用于抗癌筛选的廉价模板组合

Journal of International Translational Medicine Pub Date : 2022-11-30 DOI:10.3390/ijtm2040043

Adedokun Oluwasegun, E. Ntungwe, Ayinde Bunyamin, L. Saraíva, S. Princiotto, P. Rijo

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引用次数: 0

摘要

在尼日利亚西南部，青菜是一种著名的蔬菜，用于制作许多当地的汤。本研究旨在通过抗氧化、细胞毒性实验和癌细胞系实验来评价甘油三酯粗馏分和溶剂馏分的抗癌特性。使用Raniceps rananninus、Saccharomyces cerevisiae和Sorghum双色模型进行细胞毒性研究，以及对人乳腺癌(MCF)、结肠癌(HCT116)和肺癌(H460)细胞系的细胞毒性研究;对DPPH的自由基清除潜力也进行了同样的测试。CTA对酿酒酵母具有非依赖性的细胞毒性，抑制菌体生长的最低浓度为31.2µg/mL(26.40±1.92%)，最高活性为250µg/mL(56.00±2.12%)。在250µg/mL浓度下，CTA的抑制率为84.80±1.97%，显著高于阴性对照DMSO的33.84±1.03% (p < 0.01)。此外，所有浓度(20-40µg/mL)的CT和CTA均记录了ranninus(蝌蚪)100%的运动性，与载体(蒸馏水)的值有显著差异p < 0.05。在CT和CTA中同样注意到浓度依赖性DPPH自由基清除潜力，浓度为20-100µg/mL。CT和CTA在20µg/mL时的抑制率最低(41.35%和32.31%)，而在100µg/mL时的抑制率最高(63.26%和41.73%)。CT显示出对所有肿瘤细胞系的细胞毒作用，与CT相比，CTA对人肺(H460)、乳腺癌(MCF-7)和结肠癌(HCT116)癌细胞的活性有所提高，IC50分别为51.69±5.13、39.16±9.21和38.52±7.65。这项研究的结果从实验上证明了在尼日利亚西南部使用甘油三籽治疗癌症的民族医学主张。

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Celosia trigyna Linn (Cucurbitaceae) Annihilate Human Breast, Colon, and Lung Cancer Cells: Combination of Cheap Template for Anticancer Screening

Celosia trigyna is a well-known vegetable used in the preparation of many indigenous soups in Southwestern Nigeria. The aim of this study was to evaluate the anticancer property of C. trigyna of crude and solvent fractions using antioxidant, cytotoxic bench-top bioassays, and cancer cell line experiments. Cytotoxicity was carried out using Raniceps ranninus, Saccharomyces cerevisiae, and Sorghum bicolor models, as well as cytotoxicity studies against human breast (MCF), colon (HCT116), and lung (H460) cancer cell lines; radical scavenging potential against DPPH was likewise performed. A concentration of nondependent cytotoxicity against S. cerevisiae was observed in CTA, with the lowest inhibition of organism growth at 31.2 µg/mL (26.40 ± 1.92%) and highest activity at 250 µg/mL (56.00 ± 2.12%). Concentration-dependent inhibition was observed in CTA with 84.80 ± 1.97% at 250 µg/mL, which is significantly different from values observed in DMSO (negative control) at 33.84 ± 1.03% at p < 0.01. Moreover, 100% motility of R. ranninus (tadpoles) was recorded for all concentrations (20–40 µg/mL) in CT and CTA, with significantly different p < 0.05 from values obtained for the vehicle (distilled water). Concentration-dependent DPPH radical scavenging potential was likewise noted both in CT and CTA at 20–100 µg/mL. The lowest inhibition was observed at 20 µg/mL (41.35% and 32.31%), while the highest was noted at 100 µg/mL (63.26% and 41.73%) for CT and CTA, respectively. CT showed cytotoxic effects against all cancer cell lines examined, with CTA exhibiting improved activity compared to CT against human lung (H460), breast (MCF-7), and colon (HCT116) cancer cell lines, with IC50 51.69 ± 5.13, 39.16 ± 9.21, and 38.52 ± 7.65, respectively. Findings from this research experimentally justify the ethnomedicinal claim of usage of C. trigyna in the treatment of cancer in southwestern Nigeria.

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来源期刊

Journal of International Translational Medicine MEDICINE, RESEARCH & EXPERIMENTAL-

自引率

0.00%

发文量

317

审稿时长

8 weeks

期刊介绍： Journal of International Translational Medicine (JITM, ISSN 2227-6394), founded in 2012, is an English academic journal published by Journal of International Translational Medicine Co., Ltd and sponsored by International Fderation of Translational Medicine. JITM is an open access journal freely serving to submit, review, publish, read and download full text and quote. JITM is a quarterly publication with the first issue published in March, 2013, and all articles published in English are compiled and edited by professional graphic designers according to the international compiling and editing standard. All members of the JITM Editorial Board are the famous international specialists in the field of translational medicine who come from twenty different countries and areas such as USA, Britain, France, Germany and so on.