Laura L. Eiberger, Carol L. Ventola, Bruce P. Wasserman
{"title":"红甜菜根中洋地黄苷稳定葡聚糖合成酶的增溶","authors":"Laura L. Eiberger, Carol L. Ventola, Bruce P. Wasserman","doi":"10.1016/0304-4211(85)90003-3","DOIUrl":null,"url":null,"abstract":"<div><p>UDP-glucose: 1,3-1,4 ß-glucan synthase was solubilized from red beet (<em>Beta vulgaris</em> L.) root microsomes by treatment with 1% digitonin at 30°C. The enzyme shows unusual stability for a solubilized glucan synthase, with full activity retention after storage for 1 week at 4°C.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(85)90003-3","citationCount":"13","resultStr":"{\"title\":\"Solubilization of a digitonin-stable glucan synthase from red beet root\",\"authors\":\"Laura L. Eiberger, Carol L. Ventola, Bruce P. Wasserman\",\"doi\":\"10.1016/0304-4211(85)90003-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>UDP-glucose: 1,3-1,4 ß-glucan synthase was solubilized from red beet (<em>Beta vulgaris</em> L.) root microsomes by treatment with 1% digitonin at 30°C. The enzyme shows unusual stability for a solubilized glucan synthase, with full activity retention after storage for 1 week at 4°C.</p></div>\",\"PeriodicalId\":20221,\"journal\":{\"name\":\"Plant Science Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1985-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0304-4211(85)90003-3\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Science Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0304421185900033\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Science Letters","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0304421185900033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Solubilization of a digitonin-stable glucan synthase from red beet root
UDP-glucose: 1,3-1,4 ß-glucan synthase was solubilized from red beet (Beta vulgaris L.) root microsomes by treatment with 1% digitonin at 30°C. The enzyme shows unusual stability for a solubilized glucan synthase, with full activity retention after storage for 1 week at 4°C.
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