绿色合成锐钛矿TiO2纳米形态的体内外毒理学评价

Q2 Engineering

Materials Research Innovations Pub Date : 2022-07-05 DOI:10.1080/14328917.2022.2094629

P. Shubha, K. Namratha, M. Gowda, Mb. Nayan, H. Manjunatha, K. Byrappa

{"title":"绿色合成锐钛矿TiO2纳米形态的体内外毒理学评价","authors":"P. Shubha, K. Namratha, M. Gowda, Mb. Nayan, H. Manjunatha, K. Byrappa","doi":"10.1080/14328917.2022.2094629","DOIUrl":null,"url":null,"abstract":"ABSTRACT The present study aims at the evaluation of in vitro and in vivo toxicities of green TiO2 nanoparticles (GS-TiO2) that are biosynthesised using hydroalcoholic extract of Ocimum sanctum leaves using microwave energy. Commercially available TiO2 NPs (CS-TiO2) were used for comparison. In vitro hemotoxicity was evaluated using haemolysis assay, and viability of fibroblast cells using WST-8 assay. In vivo toxicity evaluation was performed using the silkworm model. Haemolysis assay showed that GS-TiO2 caused acceptable haemolysis of 2.3% and CS-TiO2 showed higher haemolysis of 4.4%. As per WST-8 assay, viability of fibroblast cells was better in groups treated with GS-TiO2 with more than 50% survival. Silkworm growth parameters were significantly improved in GS-TiO2-treated batches. At the biomolecular level, changes in haemolymph protein banding patterns indicated strong growth promoting potential of GS-TiO2. Considering the salient findings derived from toxicity results, we propose that GS-TiO2 shall be potent NPs for various biomedical applications.","PeriodicalId":18235,"journal":{"name":"Materials Research Innovations","volume":"33 1","pages":"138 - 144"},"PeriodicalIF":0.0000,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"In vitro and in vivo toxicological evaluation of green synthesised anatase TiO2 nanoforms\",\"authors\":\"P. Shubha, K. Namratha, M. Gowda, Mb. Nayan, H. Manjunatha, K. Byrappa\",\"doi\":\"10.1080/14328917.2022.2094629\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT The present study aims at the evaluation of in vitro and in vivo toxicities of green TiO2 nanoparticles (GS-TiO2) that are biosynthesised using hydroalcoholic extract of Ocimum sanctum leaves using microwave energy. Commercially available TiO2 NPs (CS-TiO2) were used for comparison. In vitro hemotoxicity was evaluated using haemolysis assay, and viability of fibroblast cells using WST-8 assay. In vivo toxicity evaluation was performed using the silkworm model. Haemolysis assay showed that GS-TiO2 caused acceptable haemolysis of 2.3% and CS-TiO2 showed higher haemolysis of 4.4%. As per WST-8 assay, viability of fibroblast cells was better in groups treated with GS-TiO2 with more than 50% survival. Silkworm growth parameters were significantly improved in GS-TiO2-treated batches. At the biomolecular level, changes in haemolymph protein banding patterns indicated strong growth promoting potential of GS-TiO2. Considering the salient findings derived from toxicity results, we propose that GS-TiO2 shall be potent NPs for various biomedical applications.\",\"PeriodicalId\":18235,\"journal\":{\"name\":\"Materials Research Innovations\",\"volume\":\"33 1\",\"pages\":\"138 - 144\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-07-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Materials Research Innovations\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/14328917.2022.2094629\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Engineering\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materials Research Innovations","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/14328917.2022.2094629","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Engineering","Score":null,"Total":0}

引用次数: 1

摘要

摘要:本研究旨在评价绿色二氧化钛纳米粒子(GS-TiO2)的体外和体内毒性，该纳米粒子是利用微波能量生物合成的。采用市售的TiO2 NPs (CS-TiO2)进行比较。采用溶血法评估体外血液毒性，采用WST-8法评估成纤维细胞活力。采用家蚕模型进行体内毒性评价。溶血实验表明，GS-TiO2溶血率为2.3%，CS-TiO2溶血率较高，为4.4%。WST-8实验结果显示，GS-TiO2处理组成纤维细胞存活率较高，存活率超过50%。gs - tio2处理批次的家蚕生长参数显著改善。在生物分子水平上，血淋巴蛋白带模式的变化表明GS-TiO2具有很强的促生长潜力。考虑到从毒性结果中得出的显著发现，我们建议GS-TiO2应该是各种生物医学应用的有效NPs。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

In vitro and in vivo toxicological evaluation of green synthesised anatase TiO2 nanoforms

ABSTRACT The present study aims at the evaluation of in vitro and in vivo toxicities of green TiO2 nanoparticles (GS-TiO2) that are biosynthesised using hydroalcoholic extract of Ocimum sanctum leaves using microwave energy. Commercially available TiO2 NPs (CS-TiO2) were used for comparison. In vitro hemotoxicity was evaluated using haemolysis assay, and viability of fibroblast cells using WST-8 assay. In vivo toxicity evaluation was performed using the silkworm model. Haemolysis assay showed that GS-TiO2 caused acceptable haemolysis of 2.3% and CS-TiO2 showed higher haemolysis of 4.4%. As per WST-8 assay, viability of fibroblast cells was better in groups treated with GS-TiO2 with more than 50% survival. Silkworm growth parameters were significantly improved in GS-TiO2-treated batches. At the biomolecular level, changes in haemolymph protein banding patterns indicated strong growth promoting potential of GS-TiO2. Considering the salient findings derived from toxicity results, we propose that GS-TiO2 shall be potent NPs for various biomedical applications.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Materials Research Innovations 工程技术-材料科学：综合

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

2.8 months

期刊介绍： Materials Research Innovations covers all areas of materials research with a particular interest in synthesis, processing, and properties from the nanoscale to the microscale to the bulk. Coverage includes all classes of material – ceramics, metals, and polymers; semiconductors and other functional materials; organic and inorganic materials – alone or in combination as composites. Innovation in composition and processing to impart special properties to bulk materials and coatings, and for innovative applications in technology, represents a strong focus. The journal attempts to balance enduring themes of science and engineering with the innovation provided by such areas of research activity.