2-(5-(1,2,4)三唑-1- il -甲基-1- 1- 2,4)三唑-3- il)-吡啶配合物铜(ii)的儿茶酚酶活性

D. Khomenko, Roman O. Doroschuk, I. Odarych, I. Raspertova, R. Lampeka
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引用次数: 0

摘要

介绍了2-(5-(1,2,4)三唑-1-基甲基- 1h -(1,2,4)三唑-3-基)吡啶和双核铜配合物的合成方法。通过x射线结构分析确定了配合物的结构。该配合物为中心对称的[Cu2(L)2(NO3)2·2H2O]·H2O二聚体。Cu-Cu距离为4.0408 (3)Å。在配合物中,配体处于去质子化状态。因此,三唑碎片在两个金属中心之间起着桥梁的作用。铜离子处于八面体环境中。赤道面由三个三唑氮原子和一个吡啶氮原子组成。轴向位置由一个水分子和一个硝酸盐离子占据。在[Cu2(L)2(NO3)2(H2O)2]·H2O配合物溶液的ESI质谱中,发现了[Cu2L2-H]+组成的双核铜粒子对应的各向同性模式。这些数据证实了溶液中双核配合物的存在。研究了2-(5-(1,2,4)三唑-1-基甲基- 1h -(1,2,4)-三唑-3-基)吡啶双核铜(II)配合物的儿茶酚氧化酶活性。以3,5-二叔丁基儿茶酚(3,5- dtbk)为模型底物,采用初速度法研究了儿茶酚酶型模型反应的动力学。在低浓度的3,5- dtbk下,初始氧化速率与底物浓度呈线性关系,对应于底物上的一级反应。然而,在较高浓度的3,5- dtbk下,依赖图是非线性的,表明催化剂与底物的饱和。在Michaelis-Menten模型的框架内解释了初始反应速率对底物浓度的依赖形式,该模型很好地描述了天然金属酶的行为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CATECHOLASE ACTIVITY OF A COPPER(II) COMPLEX WITH THE 2-(5-(1,2,4)TRIAZOLE-1-ILMETHYL-1H-(1,2,4)-TRIAZOLE-3-IL)-PYRIDYL
Methods of the synthesis of 2-(5-(1,2,4)triazol-1-ylmethyl-1H-(1,2,4)-triazol-3-yl)-pyridine and a binuclear copper complex are described. The structure of the complex is established by X-ray structural analysis. The complex is a centrosymmetric [Cu2(L)2(NO3)2·2H2O]·H2O dimer. The Cu-Cu distance is 4.0408 (3) Å. In the complex the ligand is in a deprotonated state. Due to this, the triazole fragment acts as a bridge between the two metal centers. Copper ions are in an octahedral environment. The equatorial plane is formed by three triazole nitrogen atoms and one pyridyl nitrogen atom. The axial positions are occupied by a water molecule and a nitrate ion. Isotropic patterns corresponding to binuclear copper particles of the [Cu2L2-H]+ composition were registered in the ESI mass spectra of the solution of the [Cu2(L)2(NO3)2(H2O)2]·H2O complex. These data confirm the presence of a binuclear complex in solution. The catecholoxidase activity of the binuclear copper (II) complex based on 2-(5-(1,2,4)triazol-1-ylmethyl-1H-(1,2,4)-triazol-3-yl)-pyridine was studied. The kinetics of model reactions of the catecholase type were investigated by the method of initial velocities using a model substrate of 3,5-di-tert-butyl catechol (3,5-DTBK). At low concentrations of 3,5-DTBK, the dependence of the initial oxidation rate on the concentration of the substrate is linear, which corresponds to the first order of the reaction on the substrate. However, the dependence graph at higher concentrations of 3,5-DTBK is nonlinear and indicates the saturation of the catalyst with the substrate. The form of the dependence of the initial reaction rate on the substrate concentration is explained within the framework of the Michaelis-Menten model, which well describes the behavior of natural metaloenzymes.
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