实施底栖大型动物下一代测序(NGS)的挑战,在海洋环境质量评价中的挑战

Gerardo J. Martí-Chillón, A. Luis, Mónica Díez-Díaz, J. Gavilá, J. R. García-March, José Tena Medialdea, F. Codoñer
{"title":"实施底栖大型动物下一代测序(NGS)的挑战,在海洋环境质量评价中的挑战","authors":"Gerardo J. Martí-Chillón, A. Luis, Mónica Díez-Díaz, J. Gavilá, J. R. García-March, José Tena Medialdea, F. Codoñer","doi":"10.3390/MOL2NET-04-06112","DOIUrl":null,"url":null,"abstract":"The Water Framework Directive 2000/60/EC regulates the environmental diagnosis of the marine ecosystem, including the evaluation of species of bioindicator macroinvertebrates present in the environment. To date, these types of determinations are carried out through the morphotaxonomic identification of the benthic macrofauna present in the samples and the calculation of associated biotic indexes, a process that is time-consuming and resource-intensive, being in some cases inaccurate due to the requirement of highly specialized human resources and the difficulty of correctly identifying certain species. In this respect, DNA barcoding techniques allow the reliable identification of organisms using DNA sequencing techniques and avoiding the disadvantages of morphotaxonomic identification. On the other hand, the recent development of New Generation DNA Sequencing techniques (NGS) has allowed the development of DNA metabarcoding, i.e. the characterization of populations of organisms present in a sample using genomic data. This paper shows the fundamental challenges to be overcome in order to establish a NGS sequencing-based assessment of the marine environmental quality.","PeriodicalId":20475,"journal":{"name":"Proceedings of MOL2NET 2018, International Conference on Multidisciplinary Sciences, 4th edition","volume":"19 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Challenges for implementing Next Generation Sequencing (NGS) of benthic macrofauna challenges, in the evaluation of marine environmental quality\",\"authors\":\"Gerardo J. Martí-Chillón, A. Luis, Mónica Díez-Díaz, J. Gavilá, J. R. García-March, José Tena Medialdea, F. Codoñer\",\"doi\":\"10.3390/MOL2NET-04-06112\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The Water Framework Directive 2000/60/EC regulates the environmental diagnosis of the marine ecosystem, including the evaluation of species of bioindicator macroinvertebrates present in the environment. To date, these types of determinations are carried out through the morphotaxonomic identification of the benthic macrofauna present in the samples and the calculation of associated biotic indexes, a process that is time-consuming and resource-intensive, being in some cases inaccurate due to the requirement of highly specialized human resources and the difficulty of correctly identifying certain species. In this respect, DNA barcoding techniques allow the reliable identification of organisms using DNA sequencing techniques and avoiding the disadvantages of morphotaxonomic identification. On the other hand, the recent development of New Generation DNA Sequencing techniques (NGS) has allowed the development of DNA metabarcoding, i.e. the characterization of populations of organisms present in a sample using genomic data. This paper shows the fundamental challenges to be overcome in order to establish a NGS sequencing-based assessment of the marine environmental quality.\",\"PeriodicalId\":20475,\"journal\":{\"name\":\"Proceedings of MOL2NET 2018, International Conference on Multidisciplinary Sciences, 4th edition\",\"volume\":\"19 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-01-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of MOL2NET 2018, International Conference on Multidisciplinary Sciences, 4th edition\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/MOL2NET-04-06112\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of MOL2NET 2018, International Conference on Multidisciplinary Sciences, 4th edition","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/MOL2NET-04-06112","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

《水框架指令2000/60/EC》规定了海洋生态系统的环境诊断,包括对环境中存在的生物指示性大型无脊椎动物物种的评估。迄今为止,这些类型的确定是通过对样品中存在的底栖大型动物的形态分类鉴定和相关生物指数的计算来进行的,这是一个耗时和资源密集型的过程,在某些情况下,由于对高度专业化的人力资源的要求和正确识别某些物种的困难而不准确。在这方面,DNA条形码技术允许使用DNA测序技术可靠地鉴定生物体,并避免形态分类鉴定的缺点。另一方面,新一代DNA测序技术(NGS)的最新发展使得DNA元条形码的发展成为可能,即利用基因组数据来表征样品中存在的生物体群体。本文指出了建立基于NGS测序的海洋环境质量评价需要克服的基本挑战。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Challenges for implementing Next Generation Sequencing (NGS) of benthic macrofauna challenges, in the evaluation of marine environmental quality
The Water Framework Directive 2000/60/EC regulates the environmental diagnosis of the marine ecosystem, including the evaluation of species of bioindicator macroinvertebrates present in the environment. To date, these types of determinations are carried out through the morphotaxonomic identification of the benthic macrofauna present in the samples and the calculation of associated biotic indexes, a process that is time-consuming and resource-intensive, being in some cases inaccurate due to the requirement of highly specialized human resources and the difficulty of correctly identifying certain species. In this respect, DNA barcoding techniques allow the reliable identification of organisms using DNA sequencing techniques and avoiding the disadvantages of morphotaxonomic identification. On the other hand, the recent development of New Generation DNA Sequencing techniques (NGS) has allowed the development of DNA metabarcoding, i.e. the characterization of populations of organisms present in a sample using genomic data. This paper shows the fundamental challenges to be overcome in order to establish a NGS sequencing-based assessment of the marine environmental quality.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信