醋酸头孢瑞克对大鼠输卵管雌激素受体表达及组织病理学的干预作用

H. Pratiwi, A. Firmawati, Diana Rahmayani Putri, A. Haryo, A. W. Wardhana
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引用次数: 0

摘要

卵巢功能减退是指卵巢异常的病理状态。卵巢异常可由调节卵巢发育的促卵泡激素(FSH)、促黄体生成素(LH)等内分泌异常引起。促性腺激素(GnRH)的刺激决定了垂体中FSH和LH的产生。具有GnRH拮抗剂作用的醋酸cetrorelix可诱导大鼠卵巢功能减退模型的建立。本研究旨在了解醋酸头孢瑞克对大鼠输卵管雌激素受体表达及组织病理学的影响。实验选用Wistar品系雌性大鼠,8-10周龄,体重150-180克,每组6只。第一组(对照组)不加醋酸头孢瑞克,第二组加醋酸头孢瑞克0.009 mg/kg BW,第三组加醋酸头孢瑞克0.0135 mg/kg BW。免疫化学法观察雌激素β受体(ERs β)表达,HE染色法观察输卵管组织病理学变化。结果表明,与使用GnRH拮抗剂相比,三个治疗组有显著差异,其中最大的是ES β雌激素受体表达减少59.2%,输卵管变薄,纤毛减少。该研究的结论是醋酸Cetrorelix作为GnRH拮抗剂能够降低雌激素受体的表达,减少纤毛的数量以及输卵管壁的粘度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interventions of Cetrorelix Acetate in Estrogen Beta Receptor Expression and Histopathology in Rats Oviduct
Ovarian hypofunction is pathologic conditions where is the ovary being abnormal. The abnormality of the ovary can be induced by the abnormality of the endocrine that regulates the development of the ovary such as, follicle-stimulating hormone (FSH) and luteinizing hormone LH. The production of FSH and LH in the pituitary is determined with the Gonadotropin hormone (GnRH) stimulation. Development of rat ovarian hypofunction models can be performed with the induction of cetrorelix acetate which has an antagonist effect of GnRH. This research was conduct to know the effect of induction of cetrorelix acetate on rat oviduct estrogen beta receptor expression and histopathology. The study used three groups of female rats (Wistar strain) 8-10 weeks old and 150-180 gram weight, each group consisting of six rats. The first group (control) without cetrorelix acetate, the second group treated with cetrorelix acetate 0.009 mg/kg BW and the third group treated with cetrorelix acetate 0.0135 mg/kg BW. Observations of estrogen beta receptor expressions (ERs β) are carried out with immunochemical methods, while observations of histopathological changes of oviduct carried out by Hematocsilin-Eosin (HE stain). The results obtained indicate a significant difference from the administration of the GnRH antagonists in the three treatment groups, among others, the largest reduction of the expression of the estrogen receptor of the ES β by 59.2%, as well as the thinning of the fallopian tubes and the reduced cilia. The conclusion of the study was Cetrorelix acetate as a GnRH antagonist capable of lowering the beta estrogen receptor expression and reducing the number of cilia as well as the viscosity of the wall lining of the fallopian tubes.
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