通过波长轴向调制光束荧光检测圆二色性。

Enantiomer Pub Date : 2002-07-01 DOI:10.1080/10242430212875
A. Yamamoto, S. Kodama, A. Matsunaga, H. Nakazawa, K. Hayakawa
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引用次数: 3

摘要

描述了一种新的装置,允许荧光检测圆二色性(FDCD)检测在传统的荧光分光光度计。FDCD是一种现象,其中手性分析物的发射强度取决于激发的极化状态。虽然FDCD是一种灵敏的手性检测技术,但使用专用的FDCD光谱仪测量FDCD存在许多困难。该装置由一个偏光棱镜和一个缓速板组成,该缓速板安装在荧光分光光度计样品室的激发光侧。在该方法中,FDCD波叠加在手性分析物的荧光激发光谱上进行检测。我们还证明了在实际样品中测定手性分析物对映体纯度的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fluorescence-detected circular dichroism by modulated beam in the wavelength axial direction.
A novel device is described that permits fluorescence-detected circular dichroism (FDCD) detection in a conventional fluorescence spectrophotometer. FDCD is a phenomenon in which the emission intensity of a chiral analyte depends on the polarization state of the excitations. Although FDCD is a sensitive chiral detection technique, measurement of FDCD by using a special CD spectrometer has involved many difficulties. The proposed device consists of a polarizing prism and a retardation plate that fit on the excitation light side in the sample compartment of the fluorescence spectrophotometer. In this method, a FDCD wave superimposed on the fluorescence excitation spectrum of a chiral analyte was detected. We have also demonstrated the possibility of the enantiomeric purity determination of chiral analytes in real samples.
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