EFFECT OF SILVER NANOPARTICLES ON LEVELS OF SERUM FSH, LH AND ESTRADIOL IN PCO-INDUCED FEMALE MICE

This study was conducted to investigate the effect of two concentrations (0.5 and 1ppm) of silver nanoparticles on levels of serum FSH, LH and E2 in PCO-induced female mice. A number(n=80) of mature female mice were intraperitoneally injected with human Chorionic Gonadotropin (hCG) at a dose of (10 IU/ mL) three days a week for two weeks in order to induce PCO. Then, these mice were divided into two groups, according to the duration of treatment (30, 45 days). Each group was subdivided to subgroups according to the substance administrated. Two subgroups were treated with (0.5 and 1ppm) of silver NPs solution by (i.p) injection twice a week and the third subgroup was treated with metformin at a dose of (1mg/day) orally three days a week. Results: there was a significant reduction (P<0.05) in serum levels of FSH in the PCO group treated with (1ppm) of silver NPs for 30 days as compared to the control. While, a significant elevation (P< 0.05) was observed in levels of E2, for the same group as compared to the control. The PCO group treated with (0.5ppm) recorded a significant reduction (P <0.05) in serum levels of E2 for the same duration. But longer duration (45 days) exhibited a significant elevation (P< 0.05) in levels serum of FSH in the PCO-induced mice treated with (0.5ppm) of silver NPs which, was similar to the effect of metformin. A significant elevation (P> 0.05) in serum levels of FSH was observed in the PCO group treated with (0.5ppm) of silver NPs along 30 days as compared to their similar group that was treated along 45 days but, non significant alteration (P> 0.05) were observed in serum levels of LH or E2 between groups of the (30 days) duration as compared to their similar groups of the second duration (45 days). Conclusion: *Corresponding Author Prof. Suzan I. Bajilan College of Nursing, Basic Science Dep. University of Baghdad, Baghdad-Iraq. Article Received on 29 July 2017, Revised on 19 August 2017, Accepted on 10 Sep. 2017 DOI: 10.20959/wjpr201711-9483 World Journal of Pharmaceutical Research SJIF Impact Factor 7.523 Volume 6, Issue 11, 113-125. Research Article ISSN 2277– 7105