{"title":"银纳米粒子对pco诱导雌性小鼠血清fish、lh和雌二醇水平的影响","authors":"Lec. Assis. Suzan I. Bajilan","doi":"10.20959/WJPR201711-9483","DOIUrl":null,"url":null,"abstract":"This study was conducted to investigate the effect of two concentrations (0.5 and 1ppm) of silver nanoparticles on levels of serum FSH, LH and E2 in PCO-induced female mice. A number(n=80) of mature female mice were intraperitoneally injected with human Chorionic Gonadotropin (hCG) at a dose of (10 IU/ mL) three days a week for two weeks in order to induce PCO. Then, these mice were divided into two groups, according to the duration of treatment (30, 45 days). Each group was subdivided to subgroups according to the substance administrated. Two subgroups were treated with (0.5 and 1ppm) of silver NPs solution by (i.p) injection twice a week and the third subgroup was treated with metformin at a dose of (1mg/day) orally three days a week. Results: there was a significant reduction (P<0.05) in serum levels of FSH in the PCO group treated with (1ppm) of silver NPs for 30 days as compared to the control. While, a significant elevation (P< 0.05) was observed in levels of E2, for the same group as compared to the control. The PCO group treated with (0.5ppm) recorded a significant reduction (P <0.05) in serum levels of E2 for the same duration. But longer duration (45 days) exhibited a significant elevation (P< 0.05) in levels serum of FSH in the PCO-induced mice treated with (0.5ppm) of silver NPs which, was similar to the effect of metformin. A significant elevation (P> 0.05) in serum levels of FSH was observed in the PCO group treated with (0.5ppm) of silver NPs along 30 days as compared to their similar group that was treated along 45 days but, non significant alteration (P> 0.05) were observed in serum levels of LH or E2 between groups of the (30 days) duration as compared to their similar groups of the second duration (45 days). Conclusion: *Corresponding Author Prof. Suzan I. Bajilan College of Nursing, Basic Science Dep. University of Baghdad, Baghdad-Iraq. Article Received on 29 July 2017, Revised on 19 August 2017, Accepted on 10 Sep. 2017 DOI: 10.20959/wjpr201711-9483 World Journal of Pharmaceutical Research SJIF Impact Factor 7.523 Volume 6, Issue 11, 113-125. 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Then, these mice were divided into two groups, according to the duration of treatment (30, 45 days). Each group was subdivided to subgroups according to the substance administrated. Two subgroups were treated with (0.5 and 1ppm) of silver NPs solution by (i.p) injection twice a week and the third subgroup was treated with metformin at a dose of (1mg/day) orally three days a week. Results: there was a significant reduction (P<0.05) in serum levels of FSH in the PCO group treated with (1ppm) of silver NPs for 30 days as compared to the control. While, a significant elevation (P< 0.05) was observed in levels of E2, for the same group as compared to the control. The PCO group treated with (0.5ppm) recorded a significant reduction (P <0.05) in serum levels of E2 for the same duration. But longer duration (45 days) exhibited a significant elevation (P< 0.05) in levels serum of FSH in the PCO-induced mice treated with (0.5ppm) of silver NPs which, was similar to the effect of metformin. A significant elevation (P> 0.05) in serum levels of FSH was observed in the PCO group treated with (0.5ppm) of silver NPs along 30 days as compared to their similar group that was treated along 45 days but, non significant alteration (P> 0.05) were observed in serum levels of LH or E2 between groups of the (30 days) duration as compared to their similar groups of the second duration (45 days). Conclusion: *Corresponding Author Prof. Suzan I. Bajilan College of Nursing, Basic Science Dep. University of Baghdad, Baghdad-Iraq. Article Received on 29 July 2017, Revised on 19 August 2017, Accepted on 10 Sep. 2017 DOI: 10.20959/wjpr201711-9483 World Journal of Pharmaceutical Research SJIF Impact Factor 7.523 Volume 6, Issue 11, 113-125. 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引用次数: 2
摘要
本实验研究了0.5和1ppm两种浓度的银纳米颗粒对pco诱导雌性小鼠血清FSH、LH和E2水平的影响。80只成熟雌性小鼠,每周3天,连续2周腹腔注射人绒毛膜促性腺激素(hCG),剂量为(10 IU/ mL)。然后,将这些小鼠根据治疗时间分为两组(30、45天)。每组按给药物质再细分为亚组。两个亚组以(0.5和1ppm) NPs银溶液(i.p)注射治疗,每周2次;第三个亚组以(1mg/天)二甲双胍口服治疗,每周3天。结果:经0.5ppm银NPs处理的PCO组30天血清FSH水平明显低于同类组45天(P < 0.05),但30天组间血清LH和E2水平与同类组45天(P> 0.05)差异不显著。结论:*通讯作者susan I. Bajilan教授,巴格达大学护理学院,基础科学系,巴格达-伊拉克。文章收于2017年7月29日,修订于2017年8月19日,接受于2017年9月10日DOI: 10.20959/wjpr201711-9483 World Journal of Pharmaceutical Research SJIF影响因子7.523卷6,第11期,113-125。研究论文ISSN 2277 - 7105
EFFECT OF SILVER NANOPARTICLES ON LEVELS OF SERUM FSH, LH AND ESTRADIOL IN PCO-INDUCED FEMALE MICE
This study was conducted to investigate the effect of two concentrations (0.5 and 1ppm) of silver nanoparticles on levels of serum FSH, LH and E2 in PCO-induced female mice. A number(n=80) of mature female mice were intraperitoneally injected with human Chorionic Gonadotropin (hCG) at a dose of (10 IU/ mL) three days a week for two weeks in order to induce PCO. Then, these mice were divided into two groups, according to the duration of treatment (30, 45 days). Each group was subdivided to subgroups according to the substance administrated. Two subgroups were treated with (0.5 and 1ppm) of silver NPs solution by (i.p) injection twice a week and the third subgroup was treated with metformin at a dose of (1mg/day) orally three days a week. Results: there was a significant reduction (P<0.05) in serum levels of FSH in the PCO group treated with (1ppm) of silver NPs for 30 days as compared to the control. While, a significant elevation (P< 0.05) was observed in levels of E2, for the same group as compared to the control. The PCO group treated with (0.5ppm) recorded a significant reduction (P <0.05) in serum levels of E2 for the same duration. But longer duration (45 days) exhibited a significant elevation (P< 0.05) in levels serum of FSH in the PCO-induced mice treated with (0.5ppm) of silver NPs which, was similar to the effect of metformin. A significant elevation (P> 0.05) in serum levels of FSH was observed in the PCO group treated with (0.5ppm) of silver NPs along 30 days as compared to their similar group that was treated along 45 days but, non significant alteration (P> 0.05) were observed in serum levels of LH or E2 between groups of the (30 days) duration as compared to their similar groups of the second duration (45 days). Conclusion: *Corresponding Author Prof. Suzan I. Bajilan College of Nursing, Basic Science Dep. University of Baghdad, Baghdad-Iraq. Article Received on 29 July 2017, Revised on 19 August 2017, Accepted on 10 Sep. 2017 DOI: 10.20959/wjpr201711-9483 World Journal of Pharmaceutical Research SJIF Impact Factor 7.523 Volume 6, Issue 11, 113-125. Research Article ISSN 2277– 7105