法菲酵母产虾青素的菌种改良

H. Nangia, Mojeer Hasan, Mohd. Azhar, P. Bhatt, B. Panda
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引用次数: 4

摘要

目的:研究不同温度下不同碳源对紫外突变体法菲红酵母细胞生长和虾青素积累的影响。重点是使该过程具有成本效益和更高的生产率。材料与方法:采用自适应菌株培养,使P. rhodozyma在较高温度(35℃)下产生最佳虾青素,提高了工艺经济性。采用广谱抗菌药物链霉素、庆大霉素和氨苄西林(200 μL)和抗真菌药物氟康唑(200 μL)培养红酵母,测定其对酵母生长和虾青素产量的影响。以大米、甘蔗汁和蔗糖为碳源,在22℃、30℃、33℃和35℃的不同发酵温度下,与葡萄糖进行虾青素产量的比较。结果:红土酵母菌对抗菌药物具有耐药性,能抑制细菌和真菌的污染。选择了较为经济的水稻作为碳源,但菌株无法利用富含淀粉的基质,导致拒收。酵母在甘蔗汁中生长时,在30°C和610 nm波长下的生物量最高(OD值2.77),而在30°C和450 nm的葡萄糖培养基中虾青素产量最高(OD值2.64)。紫外突变体的代谢和生长比较结果表明,与野生菌株相比,紫外突变体的生物量和虾青素产量显著提高。结论:在30°C条件下,含葡萄糖培养基是虾青素产率最高的碳源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Strain improvement of Phaffia rhodozyma for astaxanthin production
Objective: The aim of the present study is to evaluate and optimize different carbon sources at different temperatures in shake flask for cell growth and astaxanthin accumulation in ultraviolet (UV) mutant Phaffia rhodozyma. The focus is to make the process cost-effective and fruitful with higher productivity. Materials and Methods: Adaptive strain development was carried out so that the P. rhodozyma can give the best astaxanthin at a higher temperature (35°C), increasing the process economy. P. rhodozyma was cultivated with three broad spectrum antibacterial drugs - streptomycin, gentamycin, and ampicillin (200 μL) and with antifungal drug - fluconazole (200 μL) to determine the effect on yeast growth and astaxanthin production. Rice, cane juice, and sucrose were selected as carbon source and compared with dextrose at different fermentation temperatures - 22°C, 30°C, 33°C, and 35°C for astaxanthin production. Results: P. rhodozyma was resistant to antibiotic drugs inhibiting bacterial and fungal contaminants. Rice being more economical was selected as carbon source, but the strain was not able to utilize starch-rich substrate leading to its rejection. When P. rhodozyma was grown in cane juice, biomass is highest (OD 2.77) at 30°C and 610 nm wavelength, whereas astaxanthin productivity is highest (OD 2.64) in dextrose media at 30°C and 450 nm. Comparative metabolic and growth results for UV mutants showed significantly higher biomass and astaxanthin productivity in comparison to wild strain. Conclusion: The most efficient carbon source in terms of penny-pinching and astaxanthin productivity was found to be dextrose containing media at 30°C.
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