亚毫微米脉冲的受激辐射损耗

2003 Conference on Lasers and Electro-Optics Europe (CLEO/Europe 2003) (IEEE Cat. No.03TH8666) Pub Date : 2003-06-22 DOI:10.1109/CLEOE.2003.1313786

V. Westphal, C. Blanca, L. Kastrup, S. Hell

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引用次数: 0

摘要

我们设计了一种新的受激发射耗尽显微镜，比常用的(5-10 ps)应用更长的脉冲(bbb100 ps)。发现较长的脉冲对荧光分子的光稳定性有积极的影响。利用超快快门，在关闭STED光束后荧光信号的恢复在100 ns和10 ms之间的时间尺度上被确定。我们研究了一些可用于生物应用的染料，例如测量pH值的变化。

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Stimulated emission depletion with sub-ns pulses

We have designed a new stimulated emission depletion microscope that applies longer STED pulses (>100 ps) than commonly used (5-10 ps). It is found that longer pulses have a positive effect on photostability of the fluorescent molecules. Utilizing ultrafast shutters, the recovery of the fluorescence signal after shutting the STED beam off was determined on timescales between 100 ns and 10 ms. We examined a number of dyes that could be used in biological applications, e.g. to measure changes in pH.

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2003 Conference on Lasers and Electro-Optics Europe (CLEO/Europe 2003) (IEEE Cat. No.03TH8666)

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