C. Naughton, W. Tourtellotte, Deborah S. Smith, J. Milbrandt
{"title":"野生型和神经生长因子诱导的基因A缺陷小鼠对去势的前列腺凋亡反应","authors":"C. Naughton, W. Tourtellotte, Deborah S. Smith, J. Milbrandt","doi":"10.1046/J.1525-1411.1999.09911.X","DOIUrl":null,"url":null,"abstract":"Objective: Nerve growth factor-induced gene A (NGFIA) is a transcription factor implicated in androgen deprivation-induced apoptosis in an androgen-sensitive prostate cell line ( 1, 2). The objective of our study was to investigate the role of NGFIA in prostate apoptosis in response to androgen ablation in a mouse animal model lacking the gene. \n \n \n \nMaterials and Methods: Wild-type mice (n = 56) and NGFIA-deficient mice (“knock-out”) (n = 16) were surgically castrated. The animals were killed at 0 (noncastrated controls), 1, 3, 5, 7, 10, 14, and 21 days after castration, and the prostates were harvested. Tissue sections were stained for morphologic analysis and quantification of apoptosis using a terminal deoxynucleotidyl transferase biotinylated deoxyuridine triphosphate nick-end labeling (TUNEL) strategy. Apoptosis was quantitatively measured by counting the number of TUNEL-positive cells/100 epithelial cells by light microscopy. The percentage of apoptosis was compared for wild-type mice versus NGFIA-deficient mice after castration at the defined time points. \n \n \n \nResults: We found a statistically significant increase in the mean percentage of prostate cell apoptosis within 7–21 days after castration in both wild-type and NGFIA-deficient mice (p 0.05). \n \n \n \nConclusion: NGFIA does not seem to play a critical role in prostate apoptosis induced by androgen ablation in this mouse model.","PeriodicalId":22947,"journal":{"name":"The open prostate cancer journal","volume":"9 1","pages":"88-92"},"PeriodicalIF":0.0000,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prostate Apoptosis in Response to Castration in Wild‐Type and Nerve Growth Factor‐Induced Gene A‐Deficient Mice\",\"authors\":\"C. Naughton, W. Tourtellotte, Deborah S. Smith, J. Milbrandt\",\"doi\":\"10.1046/J.1525-1411.1999.09911.X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: Nerve growth factor-induced gene A (NGFIA) is a transcription factor implicated in androgen deprivation-induced apoptosis in an androgen-sensitive prostate cell line ( 1, 2). The objective of our study was to investigate the role of NGFIA in prostate apoptosis in response to androgen ablation in a mouse animal model lacking the gene. \\n \\n \\n \\nMaterials and Methods: Wild-type mice (n = 56) and NGFIA-deficient mice (“knock-out”) (n = 16) were surgically castrated. The animals were killed at 0 (noncastrated controls), 1, 3, 5, 7, 10, 14, and 21 days after castration, and the prostates were harvested. Tissue sections were stained for morphologic analysis and quantification of apoptosis using a terminal deoxynucleotidyl transferase biotinylated deoxyuridine triphosphate nick-end labeling (TUNEL) strategy. Apoptosis was quantitatively measured by counting the number of TUNEL-positive cells/100 epithelial cells by light microscopy. The percentage of apoptosis was compared for wild-type mice versus NGFIA-deficient mice after castration at the defined time points. \\n \\n \\n \\nResults: We found a statistically significant increase in the mean percentage of prostate cell apoptosis within 7–21 days after castration in both wild-type and NGFIA-deficient mice (p 0.05). \\n \\n \\n \\nConclusion: NGFIA does not seem to play a critical role in prostate apoptosis induced by androgen ablation in this mouse model.\",\"PeriodicalId\":22947,\"journal\":{\"name\":\"The open prostate cancer journal\",\"volume\":\"9 1\",\"pages\":\"88-92\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The open prostate cancer journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1046/J.1525-1411.1999.09911.X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The open prostate cancer journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1046/J.1525-1411.1999.09911.X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Prostate Apoptosis in Response to Castration in Wild‐Type and Nerve Growth Factor‐Induced Gene A‐Deficient Mice
Objective: Nerve growth factor-induced gene A (NGFIA) is a transcription factor implicated in androgen deprivation-induced apoptosis in an androgen-sensitive prostate cell line ( 1, 2). The objective of our study was to investigate the role of NGFIA in prostate apoptosis in response to androgen ablation in a mouse animal model lacking the gene.
Materials and Methods: Wild-type mice (n = 56) and NGFIA-deficient mice (“knock-out”) (n = 16) were surgically castrated. The animals were killed at 0 (noncastrated controls), 1, 3, 5, 7, 10, 14, and 21 days after castration, and the prostates were harvested. Tissue sections were stained for morphologic analysis and quantification of apoptosis using a terminal deoxynucleotidyl transferase biotinylated deoxyuridine triphosphate nick-end labeling (TUNEL) strategy. Apoptosis was quantitatively measured by counting the number of TUNEL-positive cells/100 epithelial cells by light microscopy. The percentage of apoptosis was compared for wild-type mice versus NGFIA-deficient mice after castration at the defined time points.
Results: We found a statistically significant increase in the mean percentage of prostate cell apoptosis within 7–21 days after castration in both wild-type and NGFIA-deficient mice (p 0.05).
Conclusion: NGFIA does not seem to play a critical role in prostate apoptosis induced by androgen ablation in this mouse model.