验证采珍木乙醇提取物的抗炎症效果

Journal of the Society of Cosmetic Scientists of Korea Pub Date : 2017-03-31 DOI:10.15230/SCSK.2017.43.1.19

이진영, 유단희, 주다혜, 김소라, 조희선, 주성현, 채정우

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引用次数: 2

摘要

研究了亚洲白芷(A. asiatica) 70%乙醇提取物对脂多糖(LPS)诱导的RAW 264.7细胞的抗炎活性和细胞活力。采用3-[4,5-二甲基噻唑-2-酰基]- 2,5 -二苯基四唑溴(MTT)法对巨噬细胞(RAW 264.7)进行细胞毒性试验，1000 μg/mL浓度下细胞存活率为96%。Griess法通过抑制LPS诱导的RAW 264.7细胞NO的产生来检测抗炎活性。结果表明，黄芪提取物对一氧化氮的产生具有浓度依赖性。western blot和逆转录聚合酶链反应(RT-PCR)证实了iNOS和COX-2蛋白的表达抑制作用。前者在500 μg/mL浓度下分别下降84.3%、56.2%，后者在500 μg/mL浓度下分别下降89.8%、84.9%。综上所述，本研究显示了积雪草提取物的抗炎作用。因此，这可以应用于抗炎剂。

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채진목 에탄올 추출물의 항염증 효과 검증

This study investigated the anti-inflammatory activities and cell viability of Amelanchier asiatica (A. asiatica) 70% ethanol extracts against RAW 264.7 cells induced by lipopolysaccharide (LPS). Cell toxicity test on macrophage cells (RAW 264.7) was performed by 3-[4,5-dimethyl-thiazol-2-yl]-2, 5-diphenyl-tetrazoliumbromide (MTT) assay and results showed 96% cell viability at 1,000 μg/mL concentration. Anti-inflammatory activity was examined via the inhibitory tests on the production of LPS induced NO in RAW 264.7 cells by Griess assay. The result showed that the extract inhibited NO production in concentration dependent manner. The iNOS and COX-2 protein expression inhibitory effects were confirmed by western blot and by reverse transcription-polymerase chain reaction (RT-PCR). From the former they were decreased by 84.3%, 56.2% at 500 μg/mL concentration, respectively, and from the latter decreased by 89.8%, 84.9% at 500 μg/mL, respectively. In conclusion, this study showed the anti-inflammatory effects of A. asiatica extracts. Thus, this could be applied to an anti-inflammatory agent.

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Journal of the Society of Cosmetic Scientists of Korea

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