考拉肝脏细胞色素p4504a、过氧化物酶体酶和烟酰胺辅助因子

S Ngo, S Kong, A Kirlich, R.A McKinnon, I Stupans
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引用次数: 29

摘要

我们检测了考拉(Phascolarctos cinereus)和袋鼠(Macropus eugenii)微粒体月桂酸羟化酶活性和氰不敏感棕榈酰辅酶A氧化活性的肝脏水平,并将我们的结果与大鼠的测定结果进行了比较。考拉微粒体月桂酸羟基化显著高于灰袋鼠和大鼠。然而,考拉体内没有对氰化物不敏感的棕榈酰辅酶a氧化。我们还确定了这些物种的肝烟酰胺辅助因子。考拉肝脏烟酰胺-腺嘌呤二核苷酸(NAD)和NAD/烟酰胺-腺嘌呤二核苷酸磷酸(NADP)比值高于灰袋鼠和大鼠肝脏。对考拉肝脏mRNA进行逆转录,然后使用基于CYP4A家族高度保守区域的引物进行聚合酶链反应,克隆出部分接近全长的cDNA克隆,核苷酸约70%,推断出的氨基酸序列与人类CYP4A11相同。该cypp被命名为CYP4A15。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytochrome P450 4A, peroxisomal enzymes and nicotinamide cofactors in koala liver

We have examined hepatic levels of microsomal lauric acid hydroxylase activity and cyanide-insensitive palmitoyl coenzyme A oxidative activity in koala (Phascolarctos cinereus) and tammar wallaby (Macropus eugenii) and compared our results to those determined in rat. Microsomal lauric acid hydroxylation was significantly higher in koala than in tammar wallaby or rat. However, cyanide-insensitive palmitoyl-CoA oxidation was absent in the koala. We have also determined the hepatic nicotinamide cofactors in these species. Hepatic nicotinamide-adenine dinucleotide (NAD) and the ratio of NAD/nicotinamide-adenine dinucleotide phosphate (NADP) were higher in koala than in tammar wallaby and rat liver. Reverse transcription of koala liver mRNA, followed by polymerase chain reaction using primers based on highly conserved areas in the CYP4A family led to the cloning of a partial, near full length, cDNA clone with ∼70% nucleotide and deduced amino acid sequence identity to human CYP4A11. The CYP has been named CYP4A15.

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