血、便临床分离大肠杆菌毒力及耐药基因的表达

Q3 Medicine

Reviews in Medical Microbiology Pub Date : 2019-07-01 DOI:10.1097/MRM.0000000000000167

A. Rajabi, H. Rajabi-vardanjani, Kobra Rastiyani, Mais E. Ahmed, Seyede Amene Mirforughi, F. Norouzi

{"title":"血、便临床分离大肠杆菌毒力及耐药基因的表达","authors":"A. Rajabi, H. Rajabi-vardanjani, Kobra Rastiyani, Mais E. Ahmed, Seyede Amene Mirforughi, F. Norouzi","doi":"10.1097/MRM.0000000000000167","DOIUrl":null,"url":null,"abstract":"Objective: As major nosocomial pathogens, Escherichia coli isolates exhibit antibiotic resistance and also express adhesive structures and antibiotic resistance genes. The objective of this study was the comparison of virulence gene expression of extended-spectrum beta-lactamase (ESBL)-producing E. coli between blood and stool samples. Methods: In this study, 20 E. coli clinical isolates (10 ESBL-producers including 5 from blood, 5 from stool samples and 10 non-ESBL-producer strains) were included. The existence of fimA, kpsMII and cdt (adhesives and toxin), acr-ab (efflux-encoding) and bla(CTX-M1) genes were confirmed by PCR. The quantitative real-time PCR was performed for evaluation of gene expression. Results: ESBL-producing E. coli isolates from stool samples could express fimA, kpsMII and cdt genes significantly higher than blood samples, whereas those isolates from blood samples significantly expressed the acr-ab (efflux-encoding) genes. In addition, the bla(CTXM1) gene was expressed among isolates from stool samples significantly higher (P = 0.022) than those from blood samples according to the analysis of variance (ANOVA) test. In addition, among non-ESBL-producers, the expression of fimA, kpsMII and cdt genes was significantly lower than ESBL-producing isolates from blood samples, but not significantly different than those from stool samples. Moreover, the expression of acr-ab genes was significantly lower than those from stool samples. Conclusion: The results exhibited that the expression of virulence genes among clinical isolates of E. coli is not the same or similar in various conditions or from various clinical origins. Thus determining the profile of gene expression in each of clinical situations can be helpful in tracking the infectious pathogens. ESBL-producing strains possibly have regulatory factors for inducing higher virulence gene expression. Copyright (C) 2019 Wolters Kluwer Health, Inc. All rights reserved.","PeriodicalId":49625,"journal":{"name":"Reviews in Medical Microbiology","volume":"45 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Expression of virulence and antimicrobial resistance genes among Escherichia coli clinical isolates from blood and stool samples\",\"authors\":\"A. Rajabi, H. Rajabi-vardanjani, Kobra Rastiyani, Mais E. Ahmed, Seyede Amene Mirforughi, F. Norouzi\",\"doi\":\"10.1097/MRM.0000000000000167\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: As major nosocomial pathogens, Escherichia coli isolates exhibit antibiotic resistance and also express adhesive structures and antibiotic resistance genes. The objective of this study was the comparison of virulence gene expression of extended-spectrum beta-lactamase (ESBL)-producing E. coli between blood and stool samples. Methods: In this study, 20 E. coli clinical isolates (10 ESBL-producers including 5 from blood, 5 from stool samples and 10 non-ESBL-producer strains) were included. The existence of fimA, kpsMII and cdt (adhesives and toxin), acr-ab (efflux-encoding) and bla(CTX-M1) genes were confirmed by PCR. The quantitative real-time PCR was performed for evaluation of gene expression. Results: ESBL-producing E. coli isolates from stool samples could express fimA, kpsMII and cdt genes significantly higher than blood samples, whereas those isolates from blood samples significantly expressed the acr-ab (efflux-encoding) genes. In addition, the bla(CTXM1) gene was expressed among isolates from stool samples significantly higher (P = 0.022) than those from blood samples according to the analysis of variance (ANOVA) test. In addition, among non-ESBL-producers, the expression of fimA, kpsMII and cdt genes was significantly lower than ESBL-producing isolates from blood samples, but not significantly different than those from stool samples. Moreover, the expression of acr-ab genes was significantly lower than those from stool samples. Conclusion: The results exhibited that the expression of virulence genes among clinical isolates of E. coli is not the same or similar in various conditions or from various clinical origins. Thus determining the profile of gene expression in each of clinical situations can be helpful in tracking the infectious pathogens. ESBL-producing strains possibly have regulatory factors for inducing higher virulence gene expression. Copyright (C) 2019 Wolters Kluwer Health, Inc. All rights reserved.\",\"PeriodicalId\":49625,\"journal\":{\"name\":\"Reviews in Medical Microbiology\",\"volume\":\"45 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reviews in Medical Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1097/MRM.0000000000000167\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reviews in Medical Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1097/MRM.0000000000000167","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

摘要

目的:大肠杆菌是主要的医院病原菌，其分离株不仅具有耐药性，而且具有粘附结构和耐药基因。本研究的目的是比较产广谱β -内酰胺酶(ESBL)大肠杆菌在血液和粪便样本中的毒力基因表达。方法:本研究共收集20株临床分离的大肠杆菌(10株产esbl，其中5株来自血液，5株来自粪便，10株非产esbl)。PCR证实了fimA、kpsMII、cdt(粘接剂和毒素)、acr-ab(外排编码)和bla(CTX-M1)基因的存在。采用实时荧光定量PCR检测基因表达情况。结果:产esbl大肠杆菌粪便分离株表达fimA、kpsMII和cdt基因显著高于血液分离株，而产esbl大肠杆菌血液分离株表达acr-ab(外排编码)基因显著高于血液分离株。方差分析(ANOVA)结果显示，粪便分离株的bla(CTXM1)基因表达量显著高于血液分离株(P = 0.022)。此外，在非esbl产生株中，fimA、kpsMII和cdt基因的表达显著低于血液样本中产生esbl的分离株，但与粪便样本中无显著差异。此外，acr-ab基因的表达显著低于粪便样本。结论:临床分离的大肠杆菌在不同条件下、不同临床来源的毒力基因表达不相同或不相似。因此，确定每个临床情况下的基因表达谱可以帮助追踪感染性病原体。产esbl菌株可能具有诱导高毒力基因表达的调控因子。版权所有2019威科健康有限公司版权所有。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Expression of virulence and antimicrobial resistance genes among Escherichia coli clinical isolates from blood and stool samples

Objective: As major nosocomial pathogens, Escherichia coli isolates exhibit antibiotic resistance and also express adhesive structures and antibiotic resistance genes. The objective of this study was the comparison of virulence gene expression of extended-spectrum beta-lactamase (ESBL)-producing E. coli between blood and stool samples. Methods: In this study, 20 E. coli clinical isolates (10 ESBL-producers including 5 from blood, 5 from stool samples and 10 non-ESBL-producer strains) were included. The existence of fimA, kpsMII and cdt (adhesives and toxin), acr-ab (efflux-encoding) and bla(CTX-M1) genes were confirmed by PCR. The quantitative real-time PCR was performed for evaluation of gene expression. Results: ESBL-producing E. coli isolates from stool samples could express fimA, kpsMII and cdt genes significantly higher than blood samples, whereas those isolates from blood samples significantly expressed the acr-ab (efflux-encoding) genes. In addition, the bla(CTXM1) gene was expressed among isolates from stool samples significantly higher (P = 0.022) than those from blood samples according to the analysis of variance (ANOVA) test. In addition, among non-ESBL-producers, the expression of fimA, kpsMII and cdt genes was significantly lower than ESBL-producing isolates from blood samples, but not significantly different than those from stool samples. Moreover, the expression of acr-ab genes was significantly lower than those from stool samples. Conclusion: The results exhibited that the expression of virulence genes among clinical isolates of E. coli is not the same or similar in various conditions or from various clinical origins. Thus determining the profile of gene expression in each of clinical situations can be helpful in tracking the infectious pathogens. ESBL-producing strains possibly have regulatory factors for inducing higher virulence gene expression. Copyright (C) 2019 Wolters Kluwer Health, Inc. All rights reserved.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Reviews in Medical Microbiology 生物-微生物学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Reviews in Medical Microbiology is a quarterly review journal which provides a balanced coverage of the whole field of medical microbiology. The Journal publishes state-of-the art reviews, mini-reviews, case presentations and original research from on-going research of the latest developments and techniques in medical microbiology, virology, mycology, parasitology, clinical microbiology, and hospital infection. In addition, PhD-Review - a platform for young researchers, and biographical Bio-Sketch articles are also considered. Reviews are concise, authoritative, and readable synthesis of the latest information on its subject, and references are limited to the fifty key sources for full reviews and twenty for mini-reviews. Reviews in Medical Microbiology is the perfect way for both qualified and trainee microbiologists, and researchers and clinicians with an interest in microbiology, to stay fully informed of the latest developments in medical microbiology. The journal is a valuable resource for educational and teaching purposes.