澳大利亚昆士兰州Oxley Creek 3-氯丙酸降解菌的分离与鉴定

A. Hadi, A. Greene
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引用次数: 0

摘要

本研究对一株能降解3-氯丙酸(3CP)的细菌进行了分离鉴定。从澳大利亚昆士兰州Oxley Creek成功分离到一株3CP降解菌,命名为菌株O1。通过16S rRNA分析以及生化和形态学检测,发现菌株O1与fususiformis Lysinibacillus具有最相似的序列特征和特征。细菌在30°C的最小培养基中生长,最大浓度为20 mM 3CP,但表现出相当缓慢的倍增时间,为92小时。最重要的是,高效液相色谱(HPLC)分析显示,梭状芽胞杆菌分离物有效降解了~ 100%有效的20mM 3CP。这是首次报道一株能够充分利用3CP作为其唯一碳源的梭状芽胞杆菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and identification of 3-chloropropionic acid degrading bacterium from Oxley Creek, Queensland, Australia
This study investigated the isolation and identification of a bacterial strain that could degrade 3-chloropropionic acid (3CP) as its sole carbon source. A 3CP degrading bacteria was successfully isolated from Oxley Creek, Queensland, Australia and designated as strain O1. Analysis of 16S rRNA as well as biochemical and morphological tests, found that strain O1 shared a sequence identity and characteristics most similar to that of Lysinibacillus fusiformis. The bacteria grew at 30°C in minimal media at a maximum concentration of 20 mM 3CP, however, exhibited a rather slow doubling time of 92 hours. Most importantly, analysis by high-performance chromatography (HPLC) revealed that the Lysinibacillus fusiformis isolate effectively degraded ∼100% of available 20mM 3CP. This is the first report detailing a Lysinibacillus fusiformis strain able to utilize 3CP as its sole carbon source competently.
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