黑胡桃树皮制剂中类黄酮含量定量测定的特点

V. Kurkin, N. I. Zimenkina
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Using differential spectrophotometry, methods for the quantitative determination of the total amount of flavonoids in terms of myricitrin in the tincture and dry extract of Juglans nigra L. bark, has been developed. It has been determined that the content of the total amount of flavonoids in terms of myricitrin in the tincture and dry extract of Juglans nigra L., is 0.84±0.07% and 12.38±0.24%, respectively. The error of a single determination of the total amount of flavonoids in terms of myricitrin in the tincture and dry extract of Juglans nigra L. bark with a confidence probability of 95%, is ± 8.91% and ± 2.10%, respectively. Methods for the quantitative determination of myricitrin in the tincture and dry extract of Juglans nigra L. bark by HPLC has been developed. The content of the dominant flavonoid – myricitrin (myricetin-3-O-α-L-rhamnopyranoside) – in the tincture and dry extract of Juglans nigra L., was 0.42±0.06% and 8.45±0.24%, respectively. 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引用次数: 1

摘要

本研究的目的是利用现代仪器分析技术(分光光度法、高效液相色谱法)建立黑核桃制剂中黄酮类化合物的定量分析方法。材料和方法。研究对象为黑胡桃树皮酊剂和干提取物,样品于2020年3月和4月在萨马拉国立医科大学(萨马拉)植物园制备;杨梅素、杨梅素的标准样品(SS)。电子光谱的登记使用分光光度计«Specord 40»(Analytik Jena,德国)进行。采用反相高效液相色谱法,在俄罗斯NPAO“Nauchpribor”公司的“Milichrom-6”微柱液相色谱仪上进行色谱分析。建立了用差示分光光度法定量测定黑胡桃树皮酊剂和干浸膏中杨梅三醇类黄酮总量的方法。测定了黑核桃酊剂和干浸膏中以杨桃苷为单位的总黄酮含量分别为0.84±0.07%和12.38±0.24%。用黑胡桃树皮酊剂和干浸出物中杨梅三醇含量测定黄酮类化合物总量的误差分别为±8.91%和±2.10%,置信概率为95%。建立了用高效液相色谱法定量测定核桃树皮酊剂和干浸膏中杨梅三苷含量的方法。杨梅苷(杨梅苷-3- o -α-L-鼠李糖吡喃苷)在黑核桃酊剂和干浸膏中的含量分别为0.42±0.06%和8.45±0.24%。测定黑核桃酊剂和干浸出物中杨桃苷含量的误差分别为±15.04%和±2.96%,置信概率为95%。所建立的方法可用于黑核桃制剂中黄酮类化合物的定量测定,为解决黑核桃制剂的标准化问题提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
FEATURES OF QUANTITATIVE ESTIMATION OF FLAVONOID CONTENT IN JUGLANS NIGRA L. BARKS PREPARATIONS
The aim of the research is the development of quantification procedures of flavonoids in Juglans nigra L. barks preparations using modern instrumental analytical techniques (spectrophotometry, high performance liquid chromatography).Materials and methods. The subjects of research were tincture and dry extract of Juglans nigra L. bark, the samples of which were prepared in March and April 2020 in the Botanical Garden of Samara State Medical University (Samara); the standard samples (SS) of myricitrin, myricetin. The registration of the electronic spectra was carried out with a spectrophotometer «Specord 40» (Analytik Jena, Germany). The chromatographic analysis was carried out by the method of reversed-phase HPLC on a microcolumn liquid chromatograph “Milichrom-6” (NPAO “Nauchpribor”, Russia).Results. Using differential spectrophotometry, methods for the quantitative determination of the total amount of flavonoids in terms of myricitrin in the tincture and dry extract of Juglans nigra L. bark, has been developed. It has been determined that the content of the total amount of flavonoids in terms of myricitrin in the tincture and dry extract of Juglans nigra L., is 0.84±0.07% and 12.38±0.24%, respectively. The error of a single determination of the total amount of flavonoids in terms of myricitrin in the tincture and dry extract of Juglans nigra L. bark with a confidence probability of 95%, is ± 8.91% and ± 2.10%, respectively. Methods for the quantitative determination of myricitrin in the tincture and dry extract of Juglans nigra L. bark by HPLC has been developed. The content of the dominant flavonoid – myricitrin (myricetin-3-O-α-L-rhamnopyranoside) – in the tincture and dry extract of Juglans nigra L., was 0.42±0.06% and 8.45±0.24%, respectively. The error of the single determination of myricitrin in the tincture and dry extract of Juglans nigra L. with a confidence probability of 95% is ± 15.04% and ±2.96%, respectively.Conclusion. The developed methods for the quantitative determination of flavonoids in the preparations of Juglans nigra L. barks L. can be used in solving the problems of standardization of Juglans nigra L. preparations.
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