衍生化对耐镉红树林真菌atroviride木霉代谢物谱的影响

Q3 Environmental Science
Siti Athirah Mohamad Jamali, Kamalrul Azlan Azizan, S. Baharum, M. Taib
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引用次数: 0

摘要

红树林真菌atroviride Trichoderma对重金属镉具有耐受性,研究其代谢产物对镉毒性的反应具有重要意义。本研究旨在探讨衍生化剂对耐镉T. atroviride代谢物数量和类型的影响,采用GC-MS分析检测。用硅基化反应和烷基化反应对经镉处理10 d的阿托维绿t细胞内和细胞外代谢产物进行衍生化反应。结果表明,当使用三种不同的衍生化剂:BSTFA, TBDMSTFA和MCF时,鉴定出更多的代谢物。通过硅基化鉴定出更多类型的代谢物,使其适用于非靶向代谢物谱研究。硅基化对于糖及其衍生物的分析是有效的,而烷基化则适用于涉及氨基酸和有机酸的针对性研究。对鉴定的代谢物数据集使用Metaboanalyst 3.0进行统计分析,然后使用偏最小二乘判别分析进行可视化。结果表明,不同类型衍生化剂之间、对照样品与镉处理样品之间的代谢物存在明显的分离。本研究使用不同衍生化剂获得了阿托维霉更全面的代谢物图谱,随后在对照和处理样品之间检测到不同的代谢物,将为未来的研究提供良好的基线信息,包括真菌对镉毒性耐受的途径和生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Derivatization on the Metabolite Profiling of the Cadmium-Tolerant Mangrove Fungus Trichoderma atroviride Using GC-MS Analysis
The mangrove fungus Trichoderma atroviride was found to be tolerant to the heavy metal cadmium and it is of high interest to profile its metabolites to gain insight into its response to cadmium toxicity. This study aimed to investigate the effect of derivatization agents on the number and types of metabolites present in the cadmium-tolerant T. atroviride, detected using GC-MS analysis. The intracellular and extracellular metabolites of T. atroviride treated with cadmium for ten days were derivatized using silylation and alkylation reactions. The results showed that a higher number of metabolites were identified when the three different derivatization agents were used: BSTFA, TBDMSTFA, and MCF. More types of metabolites were identified by silylation, making it suitable for non-targeted metabolites profiling study.  Silylation is efficient for the analysis of sugars and their derivatives while alkylation is suitable for a targeted study involving amino acids and organic acids. Statistical analysis for the data set of identified metabolites was performed using Metaboanalyst 3.0 followed by visualization using Partial Least Square-Discriminant Analysis. The plots showed clear separations of metabolites between the different types of derivatization agents and between control and cadmium-treated samples. A more comprehensive metabolite profile of T. atroviride obtained using different derivatization agents in this study, followed by distinct metabolites detected between control and treated samples, will provide good baseline information for future investigations including the pathways and biomarkers responsible for the fungal tolerance to cadmium toxicity.
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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