TRPM8离子通道在脾细胞抗原结合功能的神经免疫调节中的作用

T. Kozyreva, L. Eliseeva, G. Khramova, Voronova Ip, A. Evtushenko
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引用次数: 1

摘要

离子通道定位于不同特化的细胞中,参与多种功能的发挥。在大鼠实验中,定量PCR显示脾细胞表达热敏TRP离子通道基因(TRPV1、TRPV2、TRPV3、TRPV4、TRPM8、TRPA1)。在体外实验中,在培养液中加入TRPM8离子通道激动剂薄荷醇(10-5 M和10-8 M),表明TRPM8存在于免疫细胞上,并对抗原结合有刺激作用。薄荷醇(1% l -薄荷醇)刺激皮肤神经传入神经中的TRPM8离子通道也对脾细胞的抗原结合功能有刺激作用,但不干扰直接暴露于脾细胞上的TRPM8激动剂对该功能的额外刺激。上述结果证实了不同定位(脾细胞和外周感觉神经元)的TRPM8离子通道在调节脾脏抗原结合功能中的重要作用。这表明神经免疫相互作用的新时刻。*通信:科日列娃电视台,生理和基础医学研究所,新西伯利亚Timakov街4号,630117,俄罗斯;新西伯利亚国立大学,新西伯利亚Pirogov街2号,630090,俄罗斯,E-mail: Kozyreva@physiol.ru
本文章由计算机程序翻译,如有差异,请以英文原文为准。
TRPM8 ion channel in neuro-immune regulation of antigen binding function of spleen cells
Ion channels, localized in cells with different specialization, may participate in the performance of various functions. In the experiments on rats, quantitative PCR has shown that spleen cells express the genes of thermosensitive TRP ion channels (TRPV1, TRPV2, TRPV3, TRPV4, TRPM8, and TRPA1). Experiments in vitro with the addition of an agonist of the TRPM8 ion channel menthol (10-5 M and 10-8 M) to the incubation medium indicate the presence of the TRPM8 on immune cells, and its stimulatory effect on the antigen binding, the number of rosette-forming cells doubles under the influence of menthol at concentration of 10-8 M. Stimulation of the TRPM8 ion channel localized in the skin nerve afferents by menthol (1% L-menthol) also has a stimulating effect on the antigen-binding function of spleen cells without interfering with the additional stimulation of this function when exposed to the TRPM8 agonist directly on spleen cells. The obtained results testify to the important role of the TRPM8 ion channel of different localization (in spleen cells and in the peripheral sensory neurons) in the regulation of the antigen binding function in the spleen. This indicates new moments of the neuro-immune interaction. *Correspondence to: Kozyreva TV, Institute of Physiology and Basic Medicine, Timakov str. 4, Novosibirsk, 630117, Russia, Novosibirsk State University, Novosibirsk, Pirogov str. 2, Novosibirsk, 630090, Russia, E-mail: Kozyreva@physiol.ru
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