尼日利亚阿布贾固体废物倾倒场细菌分离株的重金属耐受性

IF 0.8 Q4 ENVIRONMENTAL STUDIES
B. Ojiego, J. Madu, O. P. Ilo, Joshua A. Odoh, E. K. Audu, Twan Ishaku, Shauibu A. Abdullahi, I. Gadzama, P. Bolorunduro, Elijah Ekah Ella, G. Ogu
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引用次数: 0

摘要

细菌具有抵抗有毒重金属的特殊生物机制。本研究调查了尼日利亚阿布贾固体废物倾倒场(Abaji、Bwari、Gosa、Gwagwalada、Kuje和Kwali)中分离的细菌对重金属的耐受潜力。利用土壤螺旋钻在0 ~ 15、15 ~ 35和35 ~ 45 cm深度随机采集土壤样本。采用培养/生化技术对分离菌株进行细菌学分析,并在营养琼脂上对不同浓度(50 ~ 400 μg/mL)的铬(Cr)、镍(Ni)和铅(Pb)进行化学分析,进行重金属耐受性试验。统计分析显示,异养细菌数量随土壤深度的增加而显著降低(p<0.05);Gosa在0 ~ 15 cm处最高(6.89 × 109 CFU/g), Kuje在30 ~ 45 cm处最低(1.32 × 103 CFU/g)。分离细菌54种,分别为变形杆菌(33.3%)、普罗维登氏菌(29.6%)、假单胞菌(16.6%)、芽孢杆菌(9.3%)、微球菌(5.5%)、大肠杆菌(2.1%)、肠杆菌(2.1%)、沙雷氏菌(2.1%)。除微球菌、肠杆菌、大肠杆菌和沙雷氏菌对≥200 μg/mL的Cr、Ni和Pb均有100%耐药,mic值分别为850 ~ 1700(假单胞菌-变形杆菌)、950 ~ 2250(假单胞菌-芽孢杆菌)和900 ~ 1750(假单胞菌-芽孢杆菌)。其中大部分细菌(24.1% ~ 38.9%)来自Gosa和Gwagwalada垃圾场。我们的研究结果表明,这些细菌可能有希望修复场地中的重金属。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Heavy Metal Tolerance of Bacterial Isolates from Solid Waste Dumping Sites in Abuja, Nigeria
Bacteria have special bio-mechanism to resist toxic heavy metals. This study investigated heavy metal tolerance potentials of bacterial isolates from solid waste dumping sites (Abaji, Bwari, Gosa, Gwagwalada, Kuje, and Kwali) in Abuja, Nigeria. Soil samples were randomly collected from each location using soil augers at depths of 0 – 15, 15 – 35 and 35 – 45 cm. They were analyzed bacteriologically using cultural/biochemical techniques and chemically by exposing the isolates to graded concentrations (50 - 400 μg/mL) of chromium (Cr), nickel (Ni) and lead (Pb) on nutrient agar for heavy metal tolerance test. Statistical analysis revealed a significant decrease (p<0.05) in the heterotrophic bacterial count with soil depth; with the highest counts (6.89 × 109 CFU/g) noted at 0 – 15 cm (Gosa) and lowest (1.32 × 103 CFU/g) found at 30 – 45 cm (Kuje). The isolated bacteria (n=54) were Proteus (33.3 %), Providencia (29.6 %), Pseudomonas (16.6 %), Bacillus (9.3 %), Micrococcus (5.5 %), Escherichia coli (2.1 %), Enterobacter (2.1 %), and Serratia (2.1 %). All these isolates except Micrococcus spp., Enterobacter spp., Escherichia coli and Serratia spp. displayed 100 % resistance to Cr, Ni and Pb at ≥ 200 μg/mL with MICs (μg/mL) being 850 – 1700 (Pseudomonas – Proteus spp.), 950 – 2250 (Pseudomonas – Bacillus spp.) and 900 – 1750 (Pseudomonas – Bacillus spp.), respectively. Majority of these bacteria (24.1 - 38.9 %) were from Gosa and Gwagwalada dumping sites. Our findings suggested these bacteria could be promising for remediation of the heavy metals in the sites.
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来源期刊
CiteScore
1.50
自引率
10.00%
发文量
39
期刊介绍: IJESD addresses matters related to environment and sustainable development, paying special attention to relevant issues in developing countries while reporting on the latest environmental trends in industrialised nations. Its range of themes encompasses ecological studies, field research, empirical work and descriptive analyses on topics such as environmental systems, environmental policies and politics, environmental legislation, environmental impact assessment, water and energy related issues, and sustainability. Other matters related to or which influence the international debate on sustainability will be also considered. Topics covered include: -Global environmental issues- Agenda 21- Energy, water, recycling, waste management- Environmental reports- Environmental policies and action plans- Environmental systems- Environmental legislation- Environmental impact assessment- Sustainable development approaches and methods.
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