利用单分子荧光时间迹反馈改善光稳定性

Annales De Physique Pub Date : 2007-01-01 DOI:10.1051/ANPHYS:2008026

J. Murray, V. Jacques, F. Marquier, D. Chauvat, F. Grosshans, F. Treussart, J. Roch

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引用次数: 0

摘要

单分子荧光实验受到光漂白的限制。一些光漂白途径涉及三重态的光激发。我们报告了一种光反馈技术，该技术在单荧光染料分子的光稳定性方面有很强的增强。这是通过实时检测量子跃迁到三重态，然后在分子保持三重态时快速关闭激光照明来实现的。

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Improvement of photostability using feedback from single-molecule fluorescence time trace

Single-molecule fluorescence experiments are limited by photobleaching. Some photobleaching pathways involve photoexcitation from the triplet state. We report an optical feedback technique that yields a strong enhancement in the photostability of single fluorescent dye molecules. This is achieved by detecting in real time quantum jumps to the triplet state and then quickly switching off laser illumination while the molecule remains in its triplet state.

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Annales De Physique 物理-物理：综合

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