吲哚乙酸对线粒体活性的调节

Igor V. Sarkissian, Robert G. McDaniel
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引用次数: 9

摘要

在吲哚乙酸的作用下,对玉米自交系鳞片线粒体进行呼吸。吲哚乙酸浓度在1.07·10 ~ 10 M左右时,对QO2N、P/N和P/O的促进作用最大;浓度在1.07·10 ~ 8 M以上时,对O2的吸收有明显抑制,对磷酸化的抑制程度较低。结果表明,线粒体活性的增强依赖于辅酶a;不添加辅酶a时,吲哚乙酸的影响较小。CoA的最有效浓度为0.1 mg/2.8 ml反应混合物。当吲哚乙酸浓度为1.07·10 ~ 10 M时,每隔3 min测得的氧吸收速率显著提高。此外,在反应混合物中加入吲哚乙酸后,线粒体活性几乎立即增强,而对照反应和高浓度吲哚乙酸(1.07·10−5 M)反应则滞后3 ~ 4 min。关于CoA与吲哚乙酸诱导的线粒体氧摄取率的关系,我们观察到两点:未添加辅酶a时,线粒体对O2的摄取速率急剧降低,线粒体对O2的摄取有7分钟的滞后。结果表明,吲哚乙酸通过影响氧化磷酸化途径的酶来增强线粒体活性。有人认为,吲哚乙酸通过作为一种变构效应剂来实现这种增强。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regulation of mitochondrial activity by indoleacetic acid

Mitochondria of scutella of a maize inbred were allowed to respire in the presence of indoleacetic acid. Greatest enhancement of QO2N, P/N and P/O was observed with indoleacetic acid concentration around 1.07 · 10−10 M. At concentrations above 1.07 · 10−8 M, O2 uptake was greatly inhibited while phosphorylation was inhibited to a lesser degree. It was shown that enhancement of mitochondrial activity is dependent on CoA; without added CoA, effects of indoleacetic acid were slight. The most effective concentration of CoA was shown to be 0.1 mg/2.8 ml reaction mixture. In the presence of 1.07 · 10−10 M indoleacetic acid, rate of O2 uptake measured at 3-min intervals was greatly increased. In addition, with indoleacetic acid in the reaction mixture, mitochondria showed an almost immediate enhancement of activity while the control reaction and the reaction with high concentration of indoleacetic acid (1.07 · 10−5 M) exhibited a lag of 3–4 min. With regard to the relationship of CoA and indoleacetic acid-induced stimulation of rate of mitochondrial O2 uptake, two points were observed: without added CoA, the rate of O2 uptake is sharply reduced and there is a lag of 7 min before mitochondria show any O2 uptake.

The results suggest that indoleacetic acid enhances mitochondrial activity by affecting the enzyme(s) of the oxidative phosphorylation pathway. It is suggested that indoleacetic acid accomplishes such enhancement by acting as an allosteric effector.

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