培养基和培养条件的优化及纳米绿藻生长各阶段主要生化成分水平的实验室变化

Journal of the Marine Biological Association of India. Marine Biological Association of India Pub Date : 2021-01-15 DOI:10.6024/jmbai.2021.63.2.2261-04

Reena V. Joseph, A. Saramma, P. Kaladharan, K. Vijayalakshmi, Abhijith Muralidharan, P. Anil, M. J. Jifriya

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引用次数: 0

摘要

本研究的主要目的是在实验室中比较几种培养条件和培养基。将无性系砧木接种到不同的培养基上，并对生物量和色素含量进行了评价。在研究的三种培养基中，发现f/2培养基产生最大的细胞密度。在f/2培养基上优化了pH、温度和盐度等培养条件，发现pH 9、温度20℃、盐度25 ppt的产量最佳。对生物化学成分的估计表明，在固定阶段脂肪含量较高，这表明了合适的收获时间，这种活饲料生物也可以考虑在未来用于生物燃料。

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Optimization of media and culture conditions and changes in the levels of major biochemical constituents during growth stages of Nannochloropsis oculata in the laboratory

Comparison of media and a few culture conditions for growing Nannochloropsis oculata, in laboratory is the major objective of this communication. Axenic stock culture was inoculated to different culture media and were evaluated for biomass production and pigment content. Of the three media studied, the f/2 medium was found to produce maximum cell density. Culture conditions like pH, temperature and salinity, optimized in f/2 medium revealed that pH 9, temperature 20°C and salinity 25 ppt were found to yield optimum output. Estimation of biochemical constituents showed more lipid content in the stationary phase, indicating the right harvesting time and this live feed organisms can also be considered for biofuel in the future.

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Journal of the Marine Biological Association of India. Marine Biological Association of India

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