Marina Antunes, Camilla Lazzaretti, G. D. Gamaro, Rafael Linden
{"title":"用2,4-二硝基苯基肼衍生化后，高效液相色谱法测定人血浆中丙二醛的预分析和验证研究","authors":"Marina Antunes, Camilla Lazzaretti, G. D. Gamaro, Rafael Linden","doi":"10.1590/S1516-93322008000200013","DOIUrl":null,"url":null,"abstract":"Malondialdehyde (MDA) is an important biomarker for the evaluation of oxidative stress status. The aim of this study was to develop a method for plasma MDA quantification by high performance liquid chromatography with diode-array detection (HPLC-DAD), following derivatization with 2,4-dinitrophenylhydrazine (DNPH), evaluating the main preanalytical variables. The calibration curve in plasma (0 to 40 µM) presented high linearity (r² = 0.998). Main validation parameters were: recovery: 78%; LOD: 0.11 µM and LOQ: 0.38 µM. The MDA values obtained in healthy volunteers (n=38) were 3.31 ± 0.38 µM (mean ± SD). Stability studies of MDA standard solution and derivatizing reagent and plasma MDA indicated that the standard solution can be stored at -20 and 4 oC, remaining stable for at least 30 days.","PeriodicalId":21193,"journal":{"name":"Revista Brasileira De Ciencias Farmaceuticas","volume":"115 20 1","pages":"279-287"},"PeriodicalIF":0.0000,"publicationDate":"2008-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"27","resultStr":"{\"title\":\"Estudo pré-analítico e de validação para determinação de malondialdeído em plasma humano por cromatografia líquida de alta eficiência, após derivatização com 2,4-dinitrofenilhidrazina\",\"authors\":\"Marina Antunes, Camilla Lazzaretti, G. D. Gamaro, Rafael Linden\",\"doi\":\"10.1590/S1516-93322008000200013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Malondialdehyde (MDA) is an important biomarker for the evaluation of oxidative stress status. The aim of this study was to develop a method for plasma MDA quantification by high performance liquid chromatography with diode-array detection (HPLC-DAD), following derivatization with 2,4-dinitrophenylhydrazine (DNPH), evaluating the main preanalytical variables. The calibration curve in plasma (0 to 40 µM) presented high linearity (r² = 0.998). Main validation parameters were: recovery: 78%; LOD: 0.11 µM and LOQ: 0.38 µM. The MDA values obtained in healthy volunteers (n=38) were 3.31 ± 0.38 µM (mean ± SD). Stability studies of MDA standard solution and derivatizing reagent and plasma MDA indicated that the standard solution can be stored at -20 and 4 oC, remaining stable for at least 30 days.\",\"PeriodicalId\":21193,\"journal\":{\"name\":\"Revista Brasileira De Ciencias Farmaceuticas\",\"volume\":\"115 20 1\",\"pages\":\"279-287\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"27\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Brasileira De Ciencias Farmaceuticas\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1590/S1516-93322008000200013\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Brasileira De Ciencias Farmaceuticas","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/S1516-93322008000200013","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Estudo pré-analítico e de validação para determinação de malondialdeído em plasma humano por cromatografia líquida de alta eficiência, após derivatização com 2,4-dinitrofenilhidrazina
Malondialdehyde (MDA) is an important biomarker for the evaluation of oxidative stress status. The aim of this study was to develop a method for plasma MDA quantification by high performance liquid chromatography with diode-array detection (HPLC-DAD), following derivatization with 2,4-dinitrophenylhydrazine (DNPH), evaluating the main preanalytical variables. The calibration curve in plasma (0 to 40 µM) presented high linearity (r² = 0.998). Main validation parameters were: recovery: 78%; LOD: 0.11 µM and LOQ: 0.38 µM. The MDA values obtained in healthy volunteers (n=38) were 3.31 ± 0.38 µM (mean ± SD). Stability studies of MDA standard solution and derivatizing reagent and plasma MDA indicated that the standard solution can be stored at -20 and 4 oC, remaining stable for at least 30 days.
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