Antidiabetic, antioxidant and in silico studies of bacterial endosymbiont inhabiting Nephelium lappaceum L.

Abstract Endophytes, notably obtaining attention, have been abided by potential origins of bioactive metabolites. In the acquaint study, endophyte was isolated from the leaves of Nephelium lappaceum L. The chosen endosymbiont was identified by 16s rRNA partial genome sequencing and investigated for their antioxidant and antidiabetic activities. A preliminary phytochemical test was comported for the affirmation of phytoconstituents in endophytic crude extract (NLM). Antioxidant activities were conducted by using 2-diphenyl-1-picrylhydrazyl (DPPH) method and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) method to screen the radical scavenging potential. The evaluation of antidiabetic activities was done by using α-amylase and α-glucosidase inhibition assay. Qualitative phytochemical test on NLM affirmed the presence of phenols, carbohydrates, alkaloids, flavonoids, steroids, mucilage and glycosides. In silico parameters were also specified for antidiabetic activities. The antioxidant assay of NLM expressed proficient antioxidant activity of IC50±SEM 1.35±0.03 µg/mL and IC50±SEM 1.47±0.03 µg/mL, for ABTS and DPPH respectively. Antidiabetic assay results evidenced dose dependent percentage inhibition of the enzyme. The results testified estimable inhibition of α-amylase (IC50±SEM 2.549±0.08 µg/mL) and α-glucosidase inhibition (IC50±SEM 2.29±0.03µg/mL) compared to the standard drug (Acarbose). In silico study divulged that the ellagic acid component present in the plant was responsible for antidiabetic activity. Thus, the study shows that NLM has a wellspring of natural source of antioxidants and antidiabetic agents and furtherance of studies on its mechanism is recommended to know detailed facts.