白莲草药理活性的筛选

Nirmala Mahendran, Priya M Rajendran, S. Kandasamy, Gobianand Kuppannan, M. Poyil, Malarvizhi Arthanari
{"title":"白莲草药理活性的筛选","authors":"Nirmala Mahendran, Priya M Rajendran, S. Kandasamy, Gobianand Kuppannan, M. Poyil, Malarvizhi Arthanari","doi":"10.25081/cb.2023.v14.8166","DOIUrl":null,"url":null,"abstract":"Padina boergesenii is a distinctive small brown algae with rounded fronds growing to a length and diameter of 04 to 06 cm (1.6 to 2.4 in). P. boergesenii is widely present in the shallow water of tropical, subtropical and warm temperate areas. The present study aimed to investigate the anti-bacterial, anti-biofilm, antioxidant, anti-inflammatory and cytotoxicity activities of crude ethyl acetate extract of P. boergesenii. Anti-bacterial activity of crude ethyl acetate extract of P. boergesenii against Gram-positive and Gram-negative bacteria was determined using the well diffusion method. MIC of P. boergesenii against biofilm was carried out by the Resazurin method. Antioxidant potential was assessed by DPPH, FRAP, and the Hydrogen peroxide scavenging method. The anti-inflammatory activity was investigated using the albumin denaturation and heat-induced hemolysis method. Cytotoxicity activity of P. boergesenii against cell line L929 was analyzed by MTT assay. The maximum zone of inhibition obtained was 23 mm for Staphylococcus aureus, followed by 21 mm for Escherichia coli. Biofilm of Enterococcus faecalis showed higher resistance (MIC= 25.00±00.00 mg/mL). Biofilm of Acinetobacter baumannii was found to be most susceptible (MIC= 06.25±00.00 mg/mL). The IC50 value for the crude ethyl acetate extract P. boergesenii was 155.5 μg/mL for the DPPH method, 1567.18 μg/mL for the FRAP method, and 3098.27 μg/mL for the H2O2 method. The results of in vitro anti-inflammatory studies exhibited IC50= 122.33 μg/mL and 2522.40 μg/mL for albumin denaturation assay and heat-induced hemolysis method respectively. The crude ethyl acetate extract of P. boergesenii showed cytotoxicity against the growth of the L929 cell line. The present study suggested that the crude ethyl acetate extract P. boergesenii has potent antibacterial, anti-biofilm, antioxidant, anti-inflammatory and cytotoxicity activities. The bioactive components present in the P. boergesenii extract can be a promising source for pharmaceuticals.","PeriodicalId":10828,"journal":{"name":"Current Botany","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Screening of Padina boergesenii for pharmacological activities\",\"authors\":\"Nirmala Mahendran, Priya M Rajendran, S. Kandasamy, Gobianand Kuppannan, M. Poyil, Malarvizhi Arthanari\",\"doi\":\"10.25081/cb.2023.v14.8166\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Padina boergesenii is a distinctive small brown algae with rounded fronds growing to a length and diameter of 04 to 06 cm (1.6 to 2.4 in). P. boergesenii is widely present in the shallow water of tropical, subtropical and warm temperate areas. The present study aimed to investigate the anti-bacterial, anti-biofilm, antioxidant, anti-inflammatory and cytotoxicity activities of crude ethyl acetate extract of P. boergesenii. Anti-bacterial activity of crude ethyl acetate extract of P. boergesenii against Gram-positive and Gram-negative bacteria was determined using the well diffusion method. MIC of P. boergesenii against biofilm was carried out by the Resazurin method. Antioxidant potential was assessed by DPPH, FRAP, and the Hydrogen peroxide scavenging method. The anti-inflammatory activity was investigated using the albumin denaturation and heat-induced hemolysis method. Cytotoxicity activity of P. boergesenii against cell line L929 was analyzed by MTT assay. The maximum zone of inhibition obtained was 23 mm for Staphylococcus aureus, followed by 21 mm for Escherichia coli. Biofilm of Enterococcus faecalis showed higher resistance (MIC= 25.00±00.00 mg/mL). Biofilm of Acinetobacter baumannii was found to be most susceptible (MIC= 06.25±00.00 mg/mL). The IC50 value for the crude ethyl acetate extract P. boergesenii was 155.5 μg/mL for the DPPH method, 1567.18 μg/mL for the FRAP method, and 3098.27 μg/mL for the H2O2 method. The results of in vitro anti-inflammatory studies exhibited IC50= 122.33 μg/mL and 2522.40 μg/mL for albumin denaturation assay and heat-induced hemolysis method respectively. The crude ethyl acetate extract of P. boergesenii showed cytotoxicity against the growth of the L929 cell line. The present study suggested that the crude ethyl acetate extract P. boergesenii has potent antibacterial, anti-biofilm, antioxidant, anti-inflammatory and cytotoxicity activities. The bioactive components present in the P. boergesenii extract can be a promising source for pharmaceuticals.\",\"PeriodicalId\":10828,\"journal\":{\"name\":\"Current Botany\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-03-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Botany\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.25081/cb.2023.v14.8166\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Botany","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25081/cb.2023.v14.8166","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

帕迪娜是一种独特的小褐藻,圆形的叶子长到04到06厘米(1.6到2.4英寸)。boergesenii广泛存在于热带、亚热带和暖温带地区的浅水中。本研究旨在研究伯氏杆菌乙酸乙酯粗提物的抗菌、抗生物膜、抗氧化、抗炎和细胞毒活性。采用孔扩散法测定了伯氏杆菌粗乙酸乙酯提取物对革兰氏阳性菌和革兰氏阴性菌的抑菌活性。采用reazurin法对伯氏杆菌对生物膜进行MIC检测。通过DPPH、FRAP和过氧化氢清除法评估其抗氧化能力。采用白蛋白变性和热溶血法研究其抗炎活性。采用MTT法分析了勃氏假单胞菌对L929细胞株的细胞毒活性。对金黄色葡萄球菌的最大抑制区为23 mm,对大肠杆菌的最大抑制区为21 mm。粪肠球菌生物膜耐药较高(MIC= 25.00±00.00 mg/mL)。鲍曼不动杆菌的生物膜最敏感(MIC= 06.25±00.00 mg/mL)。DPPH法、FRAP法和H2O2法对粗乙酸乙酯提取物的IC50分别为155.5 μg/mL、1567.18 μg/mL和3098.27 μg/mL。体外抗炎实验结果显示,白蛋白变性法和热溶血法的IC50分别为122.33 μg/mL和2522.40 μg/mL。粗乙酸乙酯提取物对L929细胞株的生长有抑制作用。本研究表明,伯氏杆菌乙酸乙酯粗提物具有较强的抗菌、抗生物膜、抗氧化、抗炎和细胞毒活性。伯氏杆菌提取物中存在的生物活性成分可以成为有前途的药物来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening of Padina boergesenii for pharmacological activities
Padina boergesenii is a distinctive small brown algae with rounded fronds growing to a length and diameter of 04 to 06 cm (1.6 to 2.4 in). P. boergesenii is widely present in the shallow water of tropical, subtropical and warm temperate areas. The present study aimed to investigate the anti-bacterial, anti-biofilm, antioxidant, anti-inflammatory and cytotoxicity activities of crude ethyl acetate extract of P. boergesenii. Anti-bacterial activity of crude ethyl acetate extract of P. boergesenii against Gram-positive and Gram-negative bacteria was determined using the well diffusion method. MIC of P. boergesenii against biofilm was carried out by the Resazurin method. Antioxidant potential was assessed by DPPH, FRAP, and the Hydrogen peroxide scavenging method. The anti-inflammatory activity was investigated using the albumin denaturation and heat-induced hemolysis method. Cytotoxicity activity of P. boergesenii against cell line L929 was analyzed by MTT assay. The maximum zone of inhibition obtained was 23 mm for Staphylococcus aureus, followed by 21 mm for Escherichia coli. Biofilm of Enterococcus faecalis showed higher resistance (MIC= 25.00±00.00 mg/mL). Biofilm of Acinetobacter baumannii was found to be most susceptible (MIC= 06.25±00.00 mg/mL). The IC50 value for the crude ethyl acetate extract P. boergesenii was 155.5 μg/mL for the DPPH method, 1567.18 μg/mL for the FRAP method, and 3098.27 μg/mL for the H2O2 method. The results of in vitro anti-inflammatory studies exhibited IC50= 122.33 μg/mL and 2522.40 μg/mL for albumin denaturation assay and heat-induced hemolysis method respectively. The crude ethyl acetate extract of P. boergesenii showed cytotoxicity against the growth of the L929 cell line. The present study suggested that the crude ethyl acetate extract P. boergesenii has potent antibacterial, anti-biofilm, antioxidant, anti-inflammatory and cytotoxicity activities. The bioactive components present in the P. boergesenii extract can be a promising source for pharmaceuticals.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信