脂肪酸、营养物质和全身振动对雄性大鼠骨组织形态学、力学性能和代谢参数的影响

H. Bahadoran, S. Salmasi, M. Karimfar, R. Shirazi, M. Asadi, M. Naghii, G. Torkaman, M. Mofid
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摘要

本研究的目的是探讨脂肪酸、营养物质的消耗和振动对大鼠骨组织形态学和力学性能以及骨代谢参数的影响。将56只雄性Wistar大鼠分为7个独立组,按以下方法治疗8周:(1)对照组(给予常规大鼠咀嚼食物和饮水);(2) RV(正常食物+振动);(3) SPM(常规食物+振动+钙+维生素D +硼);(4) CA (SPM +菜籽油);(5) SU (SPM +葵花油);(6) CA + SU (SPM +菜籽油+葵花油);(7) CO (SPM +椰子油)。8周后,分析血浆样本的维生素D、甲状旁腺激素、降钙素、睾酮、游离睾酮和雌激素水平。切除右侧股骨进行组织形态学和几何分析。左股骨和第五腰椎也被用来评估机械性能。结果表明,与对照组相比,各治疗组大鼠骨致密面积、骨细胞数量和最大负荷能量均显著增加(P<0.05)。与对照组、RV组及两组相比,SPM组股骨小梁分离面积、股骨颈最大负荷、股骨颈刚度下降均有统计学意义(P<0.05)。对照组和不同治疗组的海绵骨和小梁分离面积、股骨颈最大负荷和刚度、腰椎最大负荷和腰椎能量对最大负荷的变化均有统计学意义(P<0.05)。CA组、CA+SU组和CO组股骨小梁分离面积较对照组减少,SU组股骨颈变形较CA组显著。最后,与其他各组相比,CO组雌激素水平显著升高。我们的研究结果表明,全身振动和椰子油的组合对骨组织形态学、力学性能和代谢参数有有益的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat
The aims of this study are to investigate the effects of consumption of fatty acids, nutrients and application of vibration on bone histomorphometry and mechanical properties in addition to metabolic bone parameters in rat. Fifty six male Wistar rats were divided into seven independent groups and treated for 8 weeks as followings: (1) Control (received regular rat chaw food and drinking water); (2) RV (regular food + vibration); (3) SPM (regular food + vibration + calcium + vitamin D + boron); (4) CA (SPM + canola oil); (5) SU (SPM + sunflower oil); (6) CA + SU (SPM + Canola oil + Sunflower oil); and (7) CO (SPM + coconut oil). After 8 weeks, plasma samples were analyzed for vitamin D, parathyroid hormone, calcitonin, testosterone, free testosterone, and estrogen levels. The right femur bone was excised for histomorphometric and geometric analyses. The left femur and fifth lumbar vertebra were also used for evaluation of mechanical properties. Our results indicated that compact bone area and number of osteocytes in all treatment groups and energy to maximal load in RV compared to control group were significantly increased (P<0.05). The decline in trabecular separation area, femoral neck maximal load, and femoral neck stiffness in SPM compared to control, RV and both groups were significant (P<0.05). Also, several significant changes in spongy bone and trabecular separation areas, femoral neck maximal load and stiffness, lumbar maximal load and lumbar energy to maximal load between control and different treatment groups were detected (P<0.05). Decrease of trabecular separation area in CA, CA+SU, and CO groups compared to control group and femoral neck deformation in SU compared to CA group were significant. Finally, significant increase in estrogen level in CO group compared to all other groups was detected. Our findings demonstrated that combination of whole body vibration and coconut oil has beneficial effects on bone histomorphometry, mechanical properties and metabolic parameters.
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