{"title":"反相高效液相色谱法测定复方富马酸喹硫平含量的建立与验证","authors":"P. Nagaraju","doi":"10.5530/PHM.2015.6.15","DOIUrl":null,"url":null,"abstract":"Objective : A simple, rapid, accurate and precise RP-HPLC method was developed for the determination of Quetiapinefumarate in pure and tablet dosage forms. Materials and Methods : Separation of the drug was achieved on aisocratic Shimadzu prominence HPLC instrument on a Waters Xterra C18 column (250x4.6 mm, 5 μ). Results : The method showed a linear response for concentration in the range of 50–150 μg/mL using buffer (9.2 ± 0.05) and acetonitrile in the ratio of 51:49 v/v with detection at 254 nm with a flow rate of 1.0 mL/min and retention time was 6.588 min. Conclusion: The method was statistically validated for linearity, accuracy, precision and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed, the %RSD from recovery studies was found to be less than 1. Key words: Quetiapinefumarate, Isocratic, C 18 , HPLC, Tablets.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"78 1","pages":"105-108"},"PeriodicalIF":0.0000,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Development and Validation of RP-HPLC Method for Estimation of Quetiapine Fumarate in Pharmaceutical Formulations\",\"authors\":\"P. Nagaraju\",\"doi\":\"10.5530/PHM.2015.6.15\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective : A simple, rapid, accurate and precise RP-HPLC method was developed for the determination of Quetiapinefumarate in pure and tablet dosage forms. Materials and Methods : Separation of the drug was achieved on aisocratic Shimadzu prominence HPLC instrument on a Waters Xterra C18 column (250x4.6 mm, 5 μ). Results : The method showed a linear response for concentration in the range of 50–150 μg/mL using buffer (9.2 ± 0.05) and acetonitrile in the ratio of 51:49 v/v with detection at 254 nm with a flow rate of 1.0 mL/min and retention time was 6.588 min. Conclusion: The method was statistically validated for linearity, accuracy, precision and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed, the %RSD from recovery studies was found to be less than 1. Key words: Quetiapinefumarate, Isocratic, C 18 , HPLC, Tablets.\",\"PeriodicalId\":19960,\"journal\":{\"name\":\"Pharmaceutical Methods\",\"volume\":\"78 1\",\"pages\":\"105-108\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-12-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmaceutical Methods\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5530/PHM.2015.6.15\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceutical Methods","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5530/PHM.2015.6.15","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Development and Validation of RP-HPLC Method for Estimation of Quetiapine Fumarate in Pharmaceutical Formulations
Objective : A simple, rapid, accurate and precise RP-HPLC method was developed for the determination of Quetiapinefumarate in pure and tablet dosage forms. Materials and Methods : Separation of the drug was achieved on aisocratic Shimadzu prominence HPLC instrument on a Waters Xterra C18 column (250x4.6 mm, 5 μ). Results : The method showed a linear response for concentration in the range of 50–150 μg/mL using buffer (9.2 ± 0.05) and acetonitrile in the ratio of 51:49 v/v with detection at 254 nm with a flow rate of 1.0 mL/min and retention time was 6.588 min. Conclusion: The method was statistically validated for linearity, accuracy, precision and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed, the %RSD from recovery studies was found to be less than 1. Key words: Quetiapinefumarate, Isocratic, C 18 , HPLC, Tablets.
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