乳胶球与巨型磷脂囊泡的粘附:静力学和动力学

Christian, Dietrich, Miglena, Angelova, Bernard, Pouligny
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引用次数: 93

摘要

我们研究了固体微球与分离的巨型脂质囊泡接触时发生的一系列现象。我们使用直径几微米的乳胶珠,通过长工作距离的光学陷阱来单独操纵它们。从~ 1 ms到~ 100 s的时间上表征了球/囊泡系统的演化。在这个时间范围内,我们确定了不同的步骤,即粘附,摄入,排出和重新捕获。在粘附步骤中,球体快速向囊泡内部移动,颗粒表面被脂质润湿。我们提出了一个简单的模型,基于脂片的粘附和拉伸之间的平衡,这解释了实验平衡配置。粘附步骤后的头/囊泡结构属于部分或完全湿润,取决于初始囊泡状态。部分润湿之后可以进行第二步,我们将其命名为颗粒摄取,这将导致颗粒表面完全(或几乎完全)润湿。摄入的特征是颗粒进一步穿透囊泡轮廓,同时囊泡大小减小。这种现象是由于膜上出现了一个动态稳定的孔,它允许最初在囊泡内的部分水流出。摄入后可以前后移动(排出和重新捕获)颗粒。在最终形态中,固体表面完全被脂质润湿,但膜与固体表面之间的接触角有限。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adhesion of Latex Spheres to Giant Phospholipid Vesicles: Statics and Dynamics
We studied the sequence of phenomena which occur when a solid microsphere is brought in contact with an isolated giant lipid vesicle. We used Latex beads, a few microns in diameter, which were manipulated individually by means of a long-working-distance optical trap. The evolution of the bead/vesicle system was characterized in time, from ∼ 1 ms to ∼ 100 s. In this time range, we identified different steps, namely adhesion, ingestion, expulsion and re-capture. In the adhesion step the sphere moves quickly in direction to the vesicle interior and the surface of the particle becomes wetted by lipids. We propose a simple model, based on the counter-balance between adhesion and stretching of the lipid lamella, which explains the experimental equilibrium configuration. The bead/vesicle configuration after the adhesion step pertains to partial or complete wetting, depending on the initial vesicle state. Partial wetting can be followed by a second step, which we named particle ingestion, and which leads to complete (or nearly complete) wetting of the particle surface. Ingestion is characterized by a further penetration of the particle across the vesicle contour, in concomitance with a decrease of the vesicle size. The phenomenon is attributed to the occurrence of a dynamically stabilized pore across the membrane, which allows part of the water initially inside the vesicle to flow out. Ingestion can be followed by a back and forth movement (expulsion and re-capture) of the particle. In the ultimate configuration, the solid surface is totally wetted by lipids, however with a finite contact angle between the membrane and the solid surface.
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