毕赤酵母中功能性甲酸脱氢酶的分泌

M. Takacs, O. Makhlynets, Patricia L. Tolbert, I. Korendovych
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引用次数: 5

摘要

生物燃料是减少二氧化碳和其他温室气体排放的重要工具。依赖于NAD+的甲酸脱氢酶已经被证明能够将二氧化碳电化学还原成甲酸,甲酸最终可以转化为甲醇。我们确定了一种功能性酶,标记为固定化,可以由毕赤酵母连续分泌。这种蛋白质可以很容易地从培养基中分离出来,并且它的活性在很长一段时间内保持不变。这是创建一个能够以最少的资源和空间生产生物燃料的自我维持系统的重要的第一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Secretion of functional formate dehydrogenase in Pichia pastoris
Biofuels are an important tool for the reduction of carbon dioxide and other greenhouse emissions. NAD+-dependent formate dehydrogenase has been previously shown to be capable of the electrochemical reduction of carbon dioxide into formate, which can be ultimately converted to methanol. We established that a functional enzyme, tagged for immobilization, could be continuously secreted by Pichia pastoris. The protein can be easily separated from the growth media and its activity remains constant over an extended period of time. This is an important first step in creating a self-sustaining system capable of producing biofuels with minimal resources and space required.
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