加入生物活性物质的v类修复材料的生物修饰

IF 0.5 Q4 DENTISTRY, ORAL SURGERY & MEDICINE
T. Binaljadm, R. Moorehead, Thafar Almela, Kirsty L. Franklin, L. Tayebi, K. Moharamzadeh
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引用次数: 6

摘要

成功修复龈下v类牙槽,需要修复材料具有特殊的生物学特性。本研究旨在评估将生物活性材料掺入玻璃离聚体水泥(GIC)对其力学和生物学性能的影响。将羟基磷灰石、壳聚糖、硫酸软骨素、生物玻璃、明胶和加工过的牛牙本质掺入到GIC修复材料中。测试了材料的抗压强度、双轴抗折强度、硬度、定型和工作时间。新材料的生物相容性通过正常口腔成纤维细胞(NOF)和TR146角质形成细胞的单层细胞培养和3d组织工程人口腔黏膜模型(3D-OMM)进行评估,采用prestoo -blue组织活力测定和组织学检查。明胶改性椎间盘的抗压强度和BFS显著降低,而硫酸软骨素改性椎间盘仅降低BFS (p值> 0.05)。对于硬度、工作时间和凝固时间,只有生物玻璃造成了工作时间的显著增加。用牛牙本质、生物玻璃和壳聚糖修饰gic后,NOF活力显著提高。组织学检查显示,除经明胶修饰的GIC外,上皮层向椎间盘间隙卷曲和生长。本研究强调了羟基磷灰石、壳聚糖、生物玻璃和牛牙本质改性GICs在龈下v类修复中的临床应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biomodification of a Class-V Restorative Material by Incorporation of Bioactive Agents
Restoring subgingival class-V cavities successfully, demand special biological properties from a restorative material. This study aimed to assess the effects of incorporating bioactive materials to glass ionomer cement (GIC) on its mechanical and biological properties. Hydroxyapatite, chitosan, chondroitin sulphate, bioglass, gelatine and processed bovine dentin were incorporated into a GIC restorative material. Compressive strength, biaxial flexural strength (BFS), hardness, setting and working time measurements were investigated. Biocompatibility of the new materials was assessed using both monolayer cell cultures of normal oral fibroblasts (NOF) and TR146 keratinocytes, and a 3D-tissue engineered human oral mucosa model (3D-OMM) using presto-blue tissue viability assay and histological examination. Significant reduction in the compressive strength and BFS of gelatine-modified discs was observed, while chondroitin sulphate-modified discs had reduced BFS only (p value > 0.05). For hardness, working and setting times, only bioglass caused significant increase in the working time. NOF viability was significantly increased when exposed to GIC-modified with bovine dentine, bioglass and chitosan. Histological examination showed curling and growth of the epithelial layer toward the disc space, except for the GIC modified with gelatine. This study has highlighted the potential for clinical application of the modified GICs with hydroxyapatite, chitosan, bioglass and bovine dentine in subgingival class-V restorations.
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来源期刊
Open Dentistry Journal
Open Dentistry Journal DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
2.00
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0.00%
发文量
86
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