{"title":"新型海洋细菌l -精氨酸脱亚胺酶体外抗增殖活性评价","authors":"Rahamat Unissa, M. Sudhakar, A. Reddy","doi":"10.9734/BMRJ/2016/23592","DOIUrl":null,"url":null,"abstract":"L-Arginine deiminase is a therapeutic l-arginine depleter found to counteract various arginine auxotrophic cancer cells (do not express ASS/OCT). The aim of the present study was to evaluate the anti -proliferative activity of the purified l-arginine deiminase from Vibrio alginolyticus 1374. Production of the enzyme was carried out by shake flask method under optimal conditions. The enzyme thus produced was purified to near homogeneity by ammonium sulphate fractionation followed by ion exchange and gel filtration chromatography. The enzyme was purified to 529.43 fold and showed final specific activity of 280.6 IU/mg with 43.5% yield. SDS-PAGE revealed that Original Research Article","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"98 1","pages":"1-10"},"PeriodicalIF":0.0000,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Evaluation of in vitro Anti-proliferative Activity of L-arginine deiminase from Novel Marine Bacterial Isolate\",\"authors\":\"Rahamat Unissa, M. Sudhakar, A. Reddy\",\"doi\":\"10.9734/BMRJ/2016/23592\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"L-Arginine deiminase is a therapeutic l-arginine depleter found to counteract various arginine auxotrophic cancer cells (do not express ASS/OCT). The aim of the present study was to evaluate the anti -proliferative activity of the purified l-arginine deiminase from Vibrio alginolyticus 1374. Production of the enzyme was carried out by shake flask method under optimal conditions. The enzyme thus produced was purified to near homogeneity by ammonium sulphate fractionation followed by ion exchange and gel filtration chromatography. The enzyme was purified to 529.43 fold and showed final specific activity of 280.6 IU/mg with 43.5% yield. SDS-PAGE revealed that Original Research Article\",\"PeriodicalId\":9269,\"journal\":{\"name\":\"British microbiology research journal\",\"volume\":\"98 1\",\"pages\":\"1-10\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-01-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"British microbiology research journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/BMRJ/2016/23592\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"British microbiology research journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/BMRJ/2016/23592","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of in vitro Anti-proliferative Activity of L-arginine deiminase from Novel Marine Bacterial Isolate
L-Arginine deiminase is a therapeutic l-arginine depleter found to counteract various arginine auxotrophic cancer cells (do not express ASS/OCT). The aim of the present study was to evaluate the anti -proliferative activity of the purified l-arginine deiminase from Vibrio alginolyticus 1374. Production of the enzyme was carried out by shake flask method under optimal conditions. The enzyme thus produced was purified to near homogeneity by ammonium sulphate fractionation followed by ion exchange and gel filtration chromatography. The enzyme was purified to 529.43 fold and showed final specific activity of 280.6 IU/mg with 43.5% yield. SDS-PAGE revealed that Original Research Article
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