Role of N-acetyl cysteine and gallic acid or their combination in Some criteria related to hepatic damage in sodium fluoride treated rats

This study was designed to investigate the ameliorative role of gallic acid (GA) and N-acetylcysteine (NAC) in reducing deleterious effect of sodium fluoride (NaF) such as, oxidative stress and hepatic dysfunction in adult male rats. Thirty adult male rats were randomly and equally divided into five groups, they were handled daily for 60 days, as follows: Control group (C), received tap water only, Sodium fluoride group (T1), received 100ppm of NaF in drinking tap water, gallic acid group (T2), rats in this group were injected intraperitoneal (i/p) 150 mg/kg/day/ of GA, N-acetylcysteine group (T3), animals in this group were administrated orally 25 mg/kg/day/of NAC, while the combination of GA and NAC were given to NaF treated group(T4) in the same previous mentioned doses and method of administration. Fasting blood sample were collected at the beginning and the end of the experiment and serum were collected for estimation of hepatic enzymes concentration and antioxidant status. After animal scarifying, samples from hepatic tissue were taken for measuring hepatic reduced glutathione (GSH)and malondialdehyde (MDA) concentration. The results showed that adminstration of NaF (T1group) caused hepatic damage manifested functionally by: significant increase in serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP) concentrations, a case of oxidative stress as explained by depression in (GSH) and elevation in MDA concentration in serum and hepatic tissue. The current result also recorded that i/p injection of GA oral administration of NAC alone or in combination with NaF caused amelioration of all previously estimated parameters.