囊性纤维化患者的混合细菌-病毒肺部感染

Q4 Medicine
Е.М.Бурмистров, К.Г.Краснослободцев, Л.Р.Аветисян, Е.А.Сиянова, О.С.Медведева, Е.Г.Целикина, Н.Б.Поляков, А.И.Соловьев, А.Ю.Воронкова, Е.И.Кондратьева, С.А.Красовский, В.Г.Жуховицкий, Е.И.Бурцева, Н.А.Никитенко, М.Ю.Чернуха, E. Burmistrov, K. Krasnoslobodtsev, L. Avetisyan, E. Siyanova, O. Medvedeva, E. Tselikina, N. Polyakov, A. Solovyev, V. Zhukhovitsky, A. Voronkova, E. Kondratyeva, S. Krasovskiy, E. Burtseva, N. Nikitenko, M. Chernukha
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引用次数: 0

摘要

囊性纤维化(CF)患者死亡的主要原因是肺部的感染过程,特别是由各种病原体引起的慢性肺部感染,最常见的是细菌、真菌或病毒的组合。来自国内和国外的混合细菌和病毒-细菌感染的数据是零碎和稀疏的。细菌性和病毒性病原体在囊性纤维化患者中的主要关联尚未得到适当的研究,并且缺乏其流行病学意义的数据。本研究的目的是评估囊性纤维化患者中细菌和病毒感染的患病率,并证实发展病毒学监测的必要性。方法。使用2006 - 2022年间CF患者(409名儿童和160名成人CF患者)呼吸道的生物材料。采用细菌学方法、分子遗传学方法(RT-PCR)和MALDI-TOF质谱法进行研究。结果。2/3的CF患者呼吸道菌群呈混合型。CF患儿肺部菌群是一个动态的微生物群落,具有高度的多样性和可变性。在成人患者中,微生物的关联比在儿童中更常见,但关联的组成不那么多样化。在我们的样本中,我们从成人患者中分离出大约40种细菌,从儿童中分离出超过85种细菌。以NFMO为主,包括洋葱伯克霍尔德菌复合物、铜绿假单胞菌、木氧化无色杆菌、鲁兰氏无色杆菌、嗜酒寡养单胞菌、金黄色葡萄球菌、白色念珠菌、曲霉等。实时荧光定量PCR显示,10%的儿童和12.9%的成人囊性纤维化患者样本中存在鼻病毒RNA。结论。我们的研究结果表明,需要持续监测CF患者的肺部微生物群,包括检测病毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mixed bacterial-viral lung infections in patients with cystic fibrosis
The main cause of death in patients with cystic fibrosis (CF) is infectious process in the lungs, in particular, chronic lung infections caused by various pathogens, most often a combination of bacteria, fungi, or viruses. Data on mixed bacterial and viral-bacterial infections from domestic and foreign sources are fragmentary and sparse. The dominant associations of bacterial and viral pathogens in patients with cystic fibrosis have not been studied properly, and data on their epidemiological significance are lacking. The aim of this study was to assess the prevalence of bacterial and viral infections in patients with cystic fibrosis and to substantiate the need for the development of virological monitoring. Methods. Biomaterials from the respiratory tract of CF patients (409 children and 160 adults with CF) examined from 2006 to 2022 were used. The study was carried out using bacteriological methods, molecular genetic methods (RT-PCR) and MALDI-TOF mass-spectrometry. Results. Microflora of the respiratory tract was shown to be mixed in 2/3 patients with CF. The microflora of the lungs of children with CF is a dynamic community of microorganisms with high diversity and variability. In adult patients, associations of microorganisms are more common than in children, but the composition of associations is less diverse. We isolated about 40 species of bacteria from adult patients and more than 85 species from children in our sample. NFMO prevailed, including Burkholderia cepacia complex, Pseudomonas aeruginosa, Achromobacter xylosoxidans, Achromobacter ruhlandii, Stenotrophomonas maltophilia, and Staphylococcus aureus, Candida albicans, Aspergillus spp. Real-time PCR showed the presence of rhinovirus RNA in 10% of samples obtained from children and 12.9% from adults with cystic fibrosis. Conclusion. Our results indicate the need for continuous monitoring of the lung microflora in patients with CF, including testing for viruses.
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来源期刊
Pulmonologiya
Pulmonologiya Medicine-Pulmonary and Respiratory Medicine
CiteScore
1.40
自引率
0.00%
发文量
70
期刊介绍: The aim of this journal is to state a scientific position of the Russian Respiratory Society (RRS) on diagnosis and treatment of respiratory diseases based on recent evidence-based clinical trial publications and international consensuses. The most important tasks of the journal are: -improvement proficiency qualifications of respiratory specialists; -education in pulmonology; -prompt publication of original studies on diagnosis and treatment of respiratory diseases; -sharing clinical experience and information about pulmonology service organization in different regions of Russia; -information on current protocols, standards and recommendations of international respiratory societies; -discussion and consequent publication Russian consensus documents and announcement of RRS activities; -publication and comments of regulatory documents of Russian Ministry of Health; -historical review of Russian pulmonology development. The scientific concept of the journal includes publication of current evidence-based studies on respiratory medicine and their discussion with the participation of Russian and foreign experts and development of national consensus documents on respiratory medicine. Russian and foreign respiratory specialists including pneumologists, TB specialists, thoracic surgeons, allergists, clinical immunologists, pediatricians, oncologists, physiologists, and therapeutists are invited to publish article in the journal.
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