黄曲霉毒素的液相色谱分析

M. Manabe, S. Matsuura
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引用次数: 0

摘要

关于黄曲霉毒素的研究报道很多,但利用液相色谱法分离黄曲霉毒素的报道很少。在前人对4种黄曲霉毒素(B1、B2、G1、G2)的液相色谱分析报道的基础上,本文对黄曲霉毒素B2a、G2a等6种黄曲霉毒素进行了液相色谱分析。考察了各种吸附剂和洗脱溶剂,采用新制备的含羧甲基吸附剂Sephadex G-10 (CM)和纯水作为洗脱溶剂,获得了良好的黄曲霉毒素相互分离效果。依次洗脱黄曲霉毒素G2a、B2a、G2、B2、G1和B1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Liquid Chromatography of Aflatoxins
There are numerous reports on studies of aflatoxins, but there are only a few reports on the isolation and mutual separation of aflatoxins by liquid chromatography. Following the previous reports on the liquid chromatography of four kinds (B1, B2, G1 and G2) of aflatoxins, the authors carried out the chromatography of six kinds of aflatoxins including aflatoxins B2a and G2a. Various kinds of adsorbents and eluting solvents were examined, and then the good mutual separation of aflatoxins was obtained by using the Sephadex G-10 (CM), a newly prepared adsorbent containing carboxymethyl group, and pure water for eluting solvent. Aflatoxins G2a, B2a, G2, B2, G1 and B1 were eluted in this order.
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