孟加拉国本地绵羊种群的遗传关系

Bangladesh Journal of Animal Science Pub Date : 2019-12-29 DOI:10.3329/bjas.v48i1.44553

G. Deb, M. Choudhury, M. Kabir, M. A. Khan, M. Ershaduzzaman, T. N. Nahar, Smj Hossain, M. Alam, M. Alim

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引用次数: 4

摘要

本研究利用微卫星标记调查了孟加拉国本地绵羊群体(Barind羊、Jamuna河流域羊、Coastal羊和Garole羊)之间的遗传关系。共采集了Barind(24只)、Jamuna河流域(24只)、Coastal(24只)、Garole(10只)和现有Chotanagpuri(10只)成年羊的96份血液样本。Chotanagpuri羊作为外群。采用QIAGEN DNA Mini提取试剂盒从血样中提取DNA，并采用纳米滴法定量。粮农组织推荐13种标记微卫星标记用于聚合酶链反应(PCR)。PCR产物经2%琼脂糖凝胶电泳确认，溴化乙锭染色显示。每个引物中等位基因的准确大小由GeneMaker V1.85 demo测定。利用Microsatellite tool kit和Dispan软件包计算等位基因频率、每个位点等位基因数、观察杂合度和期望杂合度、遗传距离(DA)。使用Dispan软件计算个体间遗传距离。这些距离值被用来构建UPGMA树。结果表明，HUJ616的平均多态性等位基因数为4个，MAF70的平均多态性等位基因数为12个。沿海羊群体的杂合度为0.54±0.04，巴林羊群体的杂合度为0.63±0.03。jaruna河流域与Barind的遗传距离最小(0.01)，Garole河流域与Costal河流域的遗传距离最大(0.17)。加洛羊和乔托纳布里羊与其他三个绵羊群体的遗传距离较高。系统发育树突图显示，贾穆纳河流域羊与巴林羊属于同一遗传群。而沿海羊、加罗尔羊和那格浦尔羊与贾穆纳河流域和沿海羊的遗传距离较高。考虑到本研究的结果，可以得出结论，Barind和Jamuna河流域羊属于一个相似的遗传群，而Garole和Coastal sheep属于两个不同的遗传群。爆炸。j .似的。科学》2019。48 (1): 17-22

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Genetic relationship among indigenous sheep population of Bangladesh

The study was conducted to investigate the genetic relationships among indigenous sheep population of Bangladesh (Barind, Jamuna river basin, Coastal and Garole sheep) using microsatellite markers. A total of 96 blood samples were collected from adult sheep of Barind (24), Jamuna River Basin (24), Coastal (24), Garole(10) and available Chotanagpuri (10) sheep. Chotanagpuri sheep was used as an outgroup population. DNA was extracted from blood samples using QIAGEN DNA Mini extraction kit and was quantified using a nanodrop. FAO recommended 13 labeled microsatellite markers were used for polymerase chain reaction (PCR). PCR product was confirmed by 2% agarose gel electrophoresis and visualized by staining with ethidium bromide.The exact allele sizes in each primer were determined by GeneMaker V1.85 demo. Microsatellite tool kit and Dispan software package were used for calculation of allele frequency, number of alleles per locus, observed and expected heterozygosity and genetic distances (DA). The Dispan software was used to calculate inter-individual genetic distances. These distance values were used to construct an UPGMA tree. Results showed that average number of polymorphic alleles per locus varied from4 in HUJ616 to 12 in MAF70. Observed heterozygosity was also varied from 0.54±0.04 in Coastal to 0.63±0.03 in Barind sheep population. Genetic distance between Jamuna river basin and Barind was lowest (0.01) and between Garole and Costal was highest (0.17). Garoleand Chotonagpuri sheep has higher genetic distance from other three sheep populations. Phylogenetic dendogram showed that sheep of Jamuna river basin and barind were belonged to same genetic group. Whereas, coastal, garole and Nagpur sheeps were shown higher genetic distances from Jamuna river basin and coastal sheep. Considering findings of this study it may be concluded that the Barind and Jamuna river basin sheep belongs to a similar genetic group while, Garole and Coastal sheep are belonging to two distinct genetic groups. Bang. J. Anim. Sci. 2019. 48 (1): 17-22

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Bangladesh Journal of Animal Science

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