盐胁迫条件下StP5CS基因过表达对菜用大豆结瘤和固氮的影响

IF 0.8 4区 生物学 Q3 AGRONOMY
Legume Research Pub Date : 2018-08-25 DOI:10.18805/LR-386
X. Ren, D. Yu, Shouping Yang, J. Gai, Yuelin Zhu
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引用次数: 5

摘要

通过石英砂培养,比较了过表达StP5CS (GenBank加入号:JN606861)的T5纯合子转基因株系(HTLs)与其野生型寄主品种(WT)的生长发育性状、菜用大豆脯氨酸(glcine max (L.))含量的差异。在盐胁迫条件下。此外,还测定了两个谷氨酰胺合成酶相关基因(gmgs1 1、gmgs1 2)、两个结瘤相关基因(GmENOD40-1、GmENOD40-2)和一个豆血红蛋白基因(GmLba)的相对表达水平。本研究旨在为阐明盐胁迫条件下转基因植物根瘤形成和根系固氮机制提供理论依据。与WT植株相比,T5 HTLs植株的株高和单株种子重显著增加,各组织中脯氨酸含量也显著升高。实时荧光定量PCR (Quantitative real-time PCR, qRT-PCR)分析显示,盐胁迫条件下T5 HTLs中gmgs1、gmgs1、gmmenod40 -1、GmENOD40-2和GmLba的表达量显著升高。这些结果表明,T5 HTLs中StP5CS的过表达促进了盐胁迫条件下转基因菜用大豆的生长、结瘤和固氮。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of StP5CS gene overexpression on nodulation and nitrogen fixation of vegetable soybean under salt stress conditions
Using a quartz sand culture, comparisons were made between the T5 homozygous transgenic lines (HTLs) that overexpress StP5CS (GenBank accession number: JN606861) and their wild-type (WT) host cultivar to examine the differences in the growth and development traits, the concentrations of proline in vegetable soybean [Glycine max (L.) Merrill] under salt stress conditions. Moreover, the relative expression levels of two glutamine synthetase-related genes (GmGS1â1, GmGS1â2), two nodulation-related genes (GmENOD40-1, GmENOD40-2), and one leghemoglobin gene (GmLba) were also measured. The purpose of this research was to provide a theoretical basis for elucidating the mechanisms of nodulation and nitrogen fixation in the roots of transgenic plants under salt stress conditions. Compared with WT plants, the plant height and seed weight per plant of T5 HTLs significantly increased, and the contents of proline in various tissues of T5 HTLs were also significantly elevated. Quantitative real-time PCR (qRT-PCR) analysis indicated that the expression levels of GmGS1â1, GmGS1â2, GmENOD40-1, GmENOD40-2, and GmLba were significantly increased in T5 HTLs under salt stress conditions. These results indicate that the overexpression of StP5CS in T5 HTLs enhanced growth, nodulation and nitrogen fixation in transgenic vegetable soybean under salt stress conditions.
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来源期刊
Legume Research
Legume Research AGRONOMY-
CiteScore
1.30
自引率
37.50%
发文量
256
审稿时长
6-12 weeks
期刊介绍: Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.
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