食品样品中生物胺测定的新方法

IF 0.5 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

Studia Universitatis Babes-bolyai Chemia Pub Date : 2017-09-30 DOI:10.24193/SUBBCHEM.2017.3.08

L. Rivoira, M. Žorž, M. Martelanc, S. Budal, Davide Carena, M. Franko, M. C. Bruzzoniti

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引用次数: 7

摘要

本文提出了新的测定葡萄酒中生物胺的分析方法。本文首次采用萘- 2,3-二甲醛(NDA)和达布西氯(DBS)对葡萄酒中BAs的衍生化进行了研究，并采用高效液相色谱-荧光(HPLC-NDA- fl)和热透镜光谱(HPLC-DBS- tls)检测器对衍生化BAs进行了分析。两种方法的检测灵敏度(lod: HPLC-NDA-FL)在27 ~ 73 μg/L;HPLC-DBS-TLS在3.4 ~ 11 μg/L范围内高于官方测定葡萄酒中生物胺的方法OIV-MA-AS315-18 (60 ~ 77 μg/L)。采用高效液相色谱- dbs - tls技术对两种白葡萄酒样品中的腐胺、尸胺、组胺和酪胺进行了分析。此外，利用Berthelot反应，利用转谷氨酰胺酶释放氨，提出了葡萄酒中生物胺的TLS快速筛选方法。这种方法使我们能够确定浓度低于0.1 mg/L的总生物量含量，以组胺当量表示。

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Novel approaches for the determination of biogenic amines in food samples

In this work, new analytical approaches for determination of biogenic amines in wines were developed. For the first time, we studied the derivatization of BAs in wines with naphthalene- 2,3-dicarboxaldehyde (NDA) and with dabsyl chloride (DBS) and analysis of derivatized BAs by HPLC coupled to fluorescence (HPLC-NDA-FL) and thermal lens spectrometry (HPLC-DBS-TLS) detectors. The sensitivity of the two methods (LODs HPLC-NDA-FL in the range 27-73 μg/L; LODs HPLC-DBS-TLS in the range 3.4-11 μg/L) was higher than that of the official method for biogenic amines in wines, OIV-MA-AS315-18 (60-77 μg/L). For its best performances, the HPLC-DBS-TLS technique was applied to the analysis of putrescine, cadaverine, histamine and tyramine in two white wine samples. Additionally, exploiting the Berthelot reaction, the TLS fast screening of biogenic amines in wines, following the release of ammonia by transglutaminase, was also proposed. This approach allowed us to determine total biogenic amount content in concentrations below 0.1 mg/L, expressed as equivalents of histamine.

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来源期刊

Studia Universitatis Babes-bolyai Chemia 化学-化学综合

CiteScore

0.80

自引率

33.30%

发文量

审稿时长

>12 weeks

期刊介绍： Studia Universitatis Babes-Bolyai, Seria Chemia publishes fundamental studies in all areas of chemistry and chemical engineering. Coverage includes experimental and theoretical reports on quantitative studies of structure and thermodynamics, kinetics, mechanisms of reactions, inorganic, organic, organometallic chemistry, biochemistry, computational chemistry, solid-state phenomena, surface chemistry, chemical technology and environmental chemistry.