选定水稻基因型花药愈伤组织诱导反应预处理潜伏期的优化

P. Sharmela, N. Ganesan, R. Saraswathi, R. Gnanam, C. Chandrasekhar
{"title":"选定水稻基因型花药愈伤组织诱导反应预处理潜伏期的优化","authors":"P. Sharmela, N. Ganesan, R. Saraswathi, R. Gnanam, C. Chandrasekhar","doi":"10.18805/ag.d-5745","DOIUrl":null,"url":null,"abstract":"Background: The microspores of five tropical japonica and five indica rice genotypes were subjected to androgenic studies. The effect of growth regulators on callus induction were studied to improve the anther culture efficiency. Methods: The cold pre-treatment of panicles at 10°C were done at different days of intervals viz., 5, 8, 10 and 12 days. The microspores at uninucleate stage were selected and dusted after pre-treatment. The anthers were cultured in N6 basal media supplemented with casein hydrolysate (250 mg/L), proline (250 mg/L), silver nitrate (100 mg/L), maltose (50 g/L) and growth regulators. Result: The 8 days of cold pre-treatment initiated calli in most of the ten genotypes. The days taken for callus induction varied with genotype from 32-55 days. The callus induction frequency ranged from 1.41 to 5.12%. The responsive genotypes (Azucena, Palawan, Nira) on callus induction were studied for their regeneration potential. Background: The microspores of five tropical japonica and five indica rice genotypes were subjected to androgenic studies. The effect of growth regulators on callus induction were studied to improve the anther culture efficiency.","PeriodicalId":7599,"journal":{"name":"Agricultural Science Digest – A Research Journal","volume":"10 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimization of Pre-treatment Incubation Period on Callus Induction Response in Anthers of Selected Rice Genotypes\",\"authors\":\"P. Sharmela, N. Ganesan, R. Saraswathi, R. Gnanam, C. Chandrasekhar\",\"doi\":\"10.18805/ag.d-5745\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: The microspores of five tropical japonica and five indica rice genotypes were subjected to androgenic studies. The effect of growth regulators on callus induction were studied to improve the anther culture efficiency. Methods: The cold pre-treatment of panicles at 10°C were done at different days of intervals viz., 5, 8, 10 and 12 days. The microspores at uninucleate stage were selected and dusted after pre-treatment. The anthers were cultured in N6 basal media supplemented with casein hydrolysate (250 mg/L), proline (250 mg/L), silver nitrate (100 mg/L), maltose (50 g/L) and growth regulators. Result: The 8 days of cold pre-treatment initiated calli in most of the ten genotypes. The days taken for callus induction varied with genotype from 32-55 days. The callus induction frequency ranged from 1.41 to 5.12%. The responsive genotypes (Azucena, Palawan, Nira) on callus induction were studied for their regeneration potential. Background: The microspores of five tropical japonica and five indica rice genotypes were subjected to androgenic studies. The effect of growth regulators on callus induction were studied to improve the anther culture efficiency.\",\"PeriodicalId\":7599,\"journal\":{\"name\":\"Agricultural Science Digest – A Research Journal\",\"volume\":\"10 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural Science Digest – A Research Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18805/ag.d-5745\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Science Digest – A Research Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18805/ag.d-5745","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

背景:对5种热带粳稻和5种籼稻基因型的小孢子进行了雄性激素研究。为了提高花药培养效率,研究了生长调节剂对愈伤组织诱导的影响。方法:在10℃条件下对穗进行不同时间间隔的冷预处理,即5、8、10、12天。预处理后选择无核期的小孢子进行除尘。花药培养在N6基础培养基中,添加酪蛋白水解物(250 mg/L)、脯氨酸(250 mg/L)、硝酸银(100 mg/L)、麦芽糖(50 g/L)和生长调节剂。结果:冷预处理8 d后,10个基因型中大部分愈伤组织发生。不同基因型的愈伤组织诱导时间为32 ~ 55 d。愈伤组织诱导频率为1.41 ~ 5.12%。研究了Azucena、Palawan、Nira对愈伤组织诱导的反应基因型。背景:对5种热带粳稻和5种籼稻基因型的小孢子进行了雄性激素研究。为了提高花药培养效率,研究了生长调节剂对愈伤组织诱导的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of Pre-treatment Incubation Period on Callus Induction Response in Anthers of Selected Rice Genotypes
Background: The microspores of five tropical japonica and five indica rice genotypes were subjected to androgenic studies. The effect of growth regulators on callus induction were studied to improve the anther culture efficiency. Methods: The cold pre-treatment of panicles at 10°C were done at different days of intervals viz., 5, 8, 10 and 12 days. The microspores at uninucleate stage were selected and dusted after pre-treatment. The anthers were cultured in N6 basal media supplemented with casein hydrolysate (250 mg/L), proline (250 mg/L), silver nitrate (100 mg/L), maltose (50 g/L) and growth regulators. Result: The 8 days of cold pre-treatment initiated calli in most of the ten genotypes. The days taken for callus induction varied with genotype from 32-55 days. The callus induction frequency ranged from 1.41 to 5.12%. The responsive genotypes (Azucena, Palawan, Nira) on callus induction were studied for their regeneration potential. Background: The microspores of five tropical japonica and five indica rice genotypes were subjected to androgenic studies. The effect of growth regulators on callus induction were studied to improve the anther culture efficiency.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
0.50
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信