超快速振荡流PCR扩增尿短串联重复序列。

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference Pub Date : 2016-01-01 DOI:10.1109/EMBC.2016.7591129

Haoyu Tang, Tao Dong

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引用次数: 0

摘要

尿中的短串联重复序列(STRs)是最敏感的体液，可作为个体鉴定的生物标志物。PCR微芯片自动化、灵敏、快速。本报告采用COMSOL结合有限元分析(Finite Element Analysis, FEA)对基底层温度分布进行研究，优化微流控芯片的结构。同时，用FTA纸和CSF1PO、TPOX、TH01 Multiplex试剂盒对从尿液中提取的DNA样本进行振荡PCR微装置的初步检测。结果表明，该方法经过27次热循环，在17分钟内完成DNA扩增，退火温度为60℃，变性温度为93℃。

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Ultrafast oscillatory-flow PCR for amplication of urine short tandem repeats.

The short tandem repeats (STRs) in human urine which is the most sensitive body fluid can be serviced as biomarkers for the individual identification. PCR microchips are automated, sensitive and rapid. In this report, COMSOL with Finite Element Analysis (FEA) is employed to study the temperature distribution of the base layer to optimize the structure of the microfluidic chip. Meanwhile, the oscillatory PCR microdevice is preliminary tested by DNA samples extracted from urine with the FTA papers and CSF1PO, TPOX, TH01 Multiplex kits. The results show that this method can finish the DNA amplification within 17 minutes for 27 thermal cycling and the annealing as well as denaturing temperature were respectively 60°C and 93 °C.

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来源期刊

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference

CiteScore

2.20

自引率

0.00%

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