Determination of the Dependence of the Spectrophotometer Zero Line Speed Deviation on the Elution Parameters Alteration

To identify dependence of the drift rate on the elution parameters alteration (in high-performance liquid chromatography), four samples of chromatograms registered by the Milichrome A-02 liquid microcolumn chromatograph were formed and compared in pairs. This chromatograph was equipped with a two-beam spectrophotometric detector with the following operating spectrum, nm: 210; 220; 230; 240; 250; 260; 280; 300. To determine dependence of the drift rate on the elution parameters, comparison was made of the two samples average drift rates, which differed only in the parameter value under study. Hypothesis about the eluate temperature alteration effect on the drift rate was confirmed at the highest level of confidence. It was established that the drift rate increased in the most (by 26.54 times) precisely with an increase in the temperature by 10 °С. Twofold increase in the eluate rate led to an increase in the average drift rate over the spectrum by 81.65 %, and the twofold increase in the detector measurement interval --- by 33.82 % (despite this, hypothesis of this relationship was taken as false). Along with the temperature, activity of the eluate particles was increasing, and the amount of energy required for the electron transition to the higher energy levels decreased. The problem of drift readings is universal and relevant for many measurement instruments. Drift is caused by systematic alteration in the metrological characteristics of the measurement tool. As a result of the detector electromagnetic radiation energy absorption by particles dissolved in the eluate, such metrological characteristic of the spectrophotometric detector as the radiation intensity changes; and it changes randomly, which in 87.5 % of cases leads to a positive drift, and in 12.5 % --- to the negative drift