锌生物强化豇豆(Vigna unguiculata L. Walp.)可溶性提取物调节体内盲肠细菌种群和肠道形态(Gallus Gallus)。

IF 3.1 4区生物学 Q2 Immunology and Microbiology

Frontiers in Bioscience-Landmark Pub Date : 2022-04-25 DOI:10.31083/j.fbl2705140

M. Gomes, H. Martino, Nikolai Kolba, J‐A. Cheng, Nikita Agarwal, M. M. Rocha, E. Tako

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引用次数: 7

摘要

生物强化是一种通过传统植物育种提高粮食作物营养价值的方法。本研究的目的是评价羊膜内给予生物强化锌和标准锌豇豆粉可溶性提取物对体内肠道功能、形态、炎症和肠道微生物群的影响。方法采用7个治疗组:(1)不注射;(2) 18 mΩ h2o;(3)菊粉50 mg/mL;(4) 50 mg/mL BRS Pajeú可溶性提取物(锌标准品);(5) 50 mg/mL BRS Aracê可溶性提取物(锌生物强化);(6) 50 mg/mL BRS Imponente可溶性提取物(锌生物强化);(7) 50 mg/mL BRS西克昔克可溶性提取物(锌生物强化)。结果与其他试验组相比，BRS改良剂组和BRS昔克昔组的梭状芽孢杆菌和大肠杆菌丰度明显降低。与菊粉和18MΩ H2O组相比，所有豇豆可溶性提取物均可增加绒毛杯状细胞总数，特别是酸性杯状细胞类型数。此外，与所有处理和对照相比，BRS Xiquexique增加了隐窝杯直径和隐窝深度。生物强化锌豇豆粉的锌含量高于锌标准粉(BRS Pajeú)，植酸锌摩尔比低于锌标准粉。总的来说，与菊粉和18MΩ H2O相比，所有豇豆可溶性提取物都保持了锌和铁吸收、刷状边界膜(BBM)功能和炎症相关蛋白的基因表达。结论:本研究表明，标准豇豆和生物强化豇豆治疗组在改善肠道形态、BBM功能、炎症和肠道微生物群方面具有潜在的营养价值，其中BRS Xiquexique可溶性提取物对改善盲肠微生物群和肠道形态的效果最大。

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Zinc Biofortified Cowpea (Vigna unguiculata L. Walp.) Soluble Extracts Modulate Assessed Cecal Bacterial Populations and Gut Morphology In Vivo (Gallus gallus).

BACKGROUND Biofortification is a method that improves the nutritional value of food crops through conventional plant breeding. The aim of this study was to evaluate the effects of intra-amniotic administration of soluble extracts from zinc (Zn) biofortified and Zn standard cowpea (Vigna unguiculata L. Walp.) flour on intestinal functionality and morphology, inflammation, and gut microbiota, in vivo. METHODS Seven treatment groups were utilized: (1) No Injection; (2) 18 MΩ H2O; (3) 50 mg/mL Inulin; (4) 50 mg/mL BRS Pajeú soluble extract (Zn standard); (5) 50 mg/mL BRS Aracê soluble extract (Zn biofortified); (6) 50 mg/mL BRS Imponente soluble extract (Zn biofortified); (7) 50 mg/mL BRS Xiquexique soluble extract (Zn biofortified). RESULTS Treatment groups with BRS Imponente and BRS Xiquexique reduced the abundance of Clostridium and E. coli when compared with all other experimental groups. All cowpea soluble extracts increased villi goblet cell number (total), specifically acidic goblet cell type number per villi relative to inulin and 18MΩ H2O groups. Moreover, BRS Xiquexique increased the crypt goblet diameter and the crypt depth compared to all treatments and controls. The Zn content in the Zn biofortified cowpea flours was higher when compared to the Zn standard flour (BRS Pajeú), and the phytate: Zn molar ratio was lower in the Zn biofortified flours compared to the Zn standard flour. In general, all cowpea soluble extracts maintained the gene expression of proteins involved with Zn and iron absorption, brush border membrane (BBM) functionality and inflammation compared to inulin and 18MΩ H2O. CONCLUSIONS This study demonstrates the potential nutritional benefit of standard and biofortified cowpea treatment groups to improve intestinal morphology, BBM functionality, inflammation, and gut microbiota, with the highest effect of BRS Xiquexique soluble extracts to improve assessed cecal microflora populations and intestinal morphology.

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来源期刊

Frontiers in Bioscience-Landmark 生物-生化与分子生物学

CiteScore

3.40

自引率

3.20%

发文量

301

审稿时长

3 months

期刊介绍： FBL is an international peer-reviewed open access journal of biological and medical science. FBL publishes state of the art advances in any discipline in the area of biology and medicine, including biochemistry and molecular biology, parasitology, virology, immunology, epidemiology, microbiology, entomology, botany, agronomy, as well as basic medicine, preventive medicine, bioinformatics and other related topics.