列维stolide A和periplogenin对胃癌细胞生长有体外和体内抑制作用

Jiaqian Guo, Hongshang Hu, S. Lee, Ji Zhong Zhao
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引用次数: 0

摘要

目的:研究天然产物列维斯托利特A (LA)和periplogenin (PPG)对胃癌细胞生长的体外抑制作用,更重要的是,在体内,单独或与治疗药物5-氟尿嘧啶(5-FU)联合使用。方法:采用甲基噻唑四唑(MTT),也称为3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑进行细胞活力研究。western blot检测caspase底物聚(adp -核糖)聚合酶(PARP)裂解和末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸(dUTP)镍端标记(TUNEL)检测细胞凋亡。以胃癌裸鼠为实验对象,检测LA的抗癌活性及其与5-FU的联合治疗效果。结果:MTT法测定两种化合物对肿瘤细胞的抑制作用呈剂量依赖性和时间依赖性。它们单独使用时可以触发细胞凋亡,而与5-FU联合使用时则更有效,通过TUNEL阳性检测和caspase底物PARP的裂解。在携带胃癌细胞的裸鼠中,单独注射LA或PPG均能抑制癌细胞的生长。其中一种化合物与5-FU联合使用的治疗比单独使用一种化合物的治疗更能抑制癌细胞的生长。结论:LA和PPG在体外和体内均可单独或与5-FU联合抑制癌细胞的生长,提示它们是有前景的治疗开发的研究药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Levistolide A and periplogenin inhibit the growth of gastric cancer cells in vitro and in vivo
Aim: In the present study, the natural products levistolide A (LA) and periplogenin (PPG) were studied for their growth inhibitory effects on the development of gastric cancer cells in vitro and, more critically, in vivo, alone or in combination with the therapeutic medication 5-fluorouracil (5-FU). Methods: Methyl thiazolyl tetrazolium (MTT), also known as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assays were used for the cell viability study. Apoptosis was detected by western blot to detect the cleavage of caspase substrate poly (ADP-ribose) polymerase (PARP) and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick-end labelling (TUNEL) assays. The nude mice bearing gastric cancer cells were used for the anti-cancer activity detection of LA and its combinational treatment effect with 5-FU. Results: The results in the present study shown that the two compounds were able to inhibit the viability of the cancer cells in a dose- and time-dependent manner by MTT method. They could trigger apoptosis when used alone, and more potently, in combination with 5-FU detected by TUNEL positivity and the cleavage of caspase substrate PARP. In nude mice bearing gastric cancer cells, injection (i.p.) of LA or PPG alone inhibited the growth of the cancer cells. The treatment using one of the compounds in combination with 5-FU inhibited the cancer cell growth at a higher level than the treatment by a compound alone. Conclusions: LA and PPG could inhibit the growth of the cancer cells, alone or in combination with 5-FU, in vitro and in vivo, suggesting that they are promising investigational drugs for therapeutic development.
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