选择性谐波光学显微镜

Imaging & Microscopy Pub Date : 2008-06-01 DOI:10.1002/IMIC.200890044

S. Chu

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引用次数: 1

摘要

SHG是厚层生物组织光学检查中重要的对比机制。纤维蛋白，如肌球蛋白和胶原蛋白，具有生物光子晶体性质，是脊椎动物中主要的SHG和声团。局部分子排列强烈影响SHG极化行为。在这里，我们展示了通过SHG偏振选择来区分肌球蛋白基肌纤维和交织的胶原肌束纤维，而不需要复杂的染色或样品/图像处理。

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Selective Harmonic Optical Microscopy

SHG is an important contrast mechanism in optical examination for thick biological tissues. Fibrous proteins, such as myosin and collagen, exhibit biophotonic crystal nature and are dominant SHG harmonophores in vertebrates. Local molecule arrangements strongly affect SHG polarization behavior. Here we demonstrate to distinguish myosin-based muscle fibers from intertwined collagenous perimysium through SHG polarization selection, without complicated staining or sample/image processing required.

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Imaging & Microscopy

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