一种新型抗蜜蜂病原体芽孢杆菌幼虫噬菌体裂解酶的分离与鉴定

L. Leblanc, S. Nezami, Diane G. Yost, Philippos K. Tsourkas, P. Amy
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引用次数: 18

摘要

拟芽孢杆菌幼虫是引起美国幼虫病(AFB)的病原体,它影响蜜蜂发育的早期幼虫阶段。由于其毒性、传播性、产生抗生素耐药性的能力以及其内生孢子的固有弹性,芽孢杆菌幼虫极难从感染的荨麻疹中根除,通常必须焚烧。蜜蜂对授粉和食物供应至关重要,而AFB导致了全球蜜蜂数量的下降。我们从一种从环境样本中提取的新型芽孢杆菌幼虫噬菌体的基因组中分离出一种新的噬菌体裂解素PlyPalA。PlyPalA具有n端n -乙酰muramyl - l-丙氨酸酰胺酶催化结构域,具有对芽孢杆菌幼虫的感染性菌株的裂解活性,而不会伤害已知组成蜜蜂幼虫微生物群的共生菌。单剂量的PlyPalA挽救了75%感染内生孢子的幼虫,表明它是未来治疗AFB的有力工具。这是首次在无脊椎动物中测试溶酶的治疗用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and characterization of a novel phage lysin active against Paenibacillus larvae, a honeybee pathogen
Paenibacillus larvae is the causative agent of American foulbrood (AFB) disease which affects early larval stages during honeybee development. Due to its virulence, transmissibility, capacity to develop antibiotic resistance, and the inherent resilience of its endospores, Paenibacillus larvae is extremely difficult to eradicate from infected hives which often must be burned. AFB contributes to the worldwide decline of honeybee populations, which are crucial for pollination and the food supply. We have isolated a novel bacteriophage lysin, PlyPalA, from the genome of a novel Paenibacillus larvae bacteriophage originally extracted from an environmental sample. PlyPalA has an N-terminal N-acetylmuramoyl-L-alanine amidase catalytic domain and possesses lytic activity against infectious strains of Paenibacillus larvae without harming commensal bacteria known to compose the honeybee larval microbiota. A single dose of PlyPalA rescued 75% of larvae infected with endospores, showing that it represents a powerful tool for future treatment of AFB. This represents the first time that lysins have been tested for therapeutic use in invertebrates.
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